OWLS 2012 - Italy

OWLS 2012
ORGANISING COMMITTEE
Alberto Diaspro, Istituto Italiano di Tecnologia and University of Genoa, Italy
Francesco Baldini, National Research Council, Florence, Italy
Paolo Bianchini, Istituto Italiano di Tecnologia, Genoa, Italy
Francesca Cella Zanacchi, Istituto Italiano di Tecnologia, Genova, Italy
Valeria Delle Cave,Istituto Italiano di Tecnologia, Genova, Italy
Zeno Lavagnino, Istituto Italiano di Tecnologia and University of Genoa, Italy
Mattia Pesce, Istituto Italiano di Tecnologia, Genova, Italy
Manuela Rigano, University of Genova, Italy
Manuela Salvatori, Istituto Italiano di Tecnologia, Genova, Italy
Federico Tixi, Istituto Italiano di Tecnologia, Genova, Italy
Cesare Usai, National Research Council, Genova, Italy
PROGRAM
Alberto Diaspro, Istituto Italiano di Tecnologia and University of Genoa, Italy
Silvio Aime, University of Turin, Italy
Francesco Baldini, National Research Council, Florence, Italy
Gert von Bally, University of Muenster, Germany
Paolo Bianchini, Istituto Italiano di Tecnologia, Genoa, Italy
Francesca Cagnoni, Istituto Italiano di Tecnologia, Genova, Italy
Halina Rubenstein-Dunlop, University of Queensland, Australia
Hans-Jochen Foth, Technical University of Kaiserslauter, Germany
Min Gu, Swinburne University, Australia
Partha Pratim Mondal, Indian Institute of Science, Bangalore, India
Stephen Morgan, University of Nottingham, UK
Colin JR Sheppard, National University of Singapore, Singapore
Cesare Usai, National Research Council, Genova, Italy
OWLS • Genoa, 4-6 July 2012 • Program • Page 1
Exhibit Area
2nd Floor
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Nikon Instruments Spa
IIT - Istituto Italiano di Tecnologia / LANIR.EU
Physik Instrumente (PI) S.r.l.
HAMAMATSU PHOTONICS ITALIA
Till Photonics GmbH
Fianium Limited
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3rd Floor
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OLYMPUS ITALIA SPA
VUTARA Inc.
TOPTICA Photonics AG
Bitplane AG
Leica Microsystems Srl
OWLS • Genoa, 4-6 July 2012 • Program • Page 3
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OWLS 2012
Tuesday, July 3rd - 7.00pm
Inaugural Ceremony
Sala del Maggior Consiglio, Palazzo Ducale
www.palazzoducale.genova.it
Alberto Diaspro, OWLS President
Roberto Cingolani, Scientific Director, Istituto Italiano di Tecnologia
“Lettura Magistrale”
Scott E. Fraser, Caltech, CA
Imaging the Molecular and Cellular Dynamics of Embryogenesis
Welcome Cocktail kindly offered by
Camera di Commercio di Genova
Leica Microsystems
Bitplane
OWLS • Genoa, 4-6 July 2012 • Program • Page 5
Wednesday, July 4th
9.00 - 9.30
Magazzini del Cotone - Congress Center
http://www.portoantico.it
Scirocco-Libeccio Room
OWLS 2012 Opening Lecture
Karsten Konig, Saarbrucken University, Germany
Clinical Multimodal Hybrid Multiphoton/CARS Tomography of In Vivo Human Skin
Chair: A. Diaspro
Parallel Sessions
9.30 - 10.00 Scirocco - Libeccio Room
David Sampson
University of Western Australia, Australia
Optical elastography - high-resolution
imaging of tissue mechanical properties.
Ponente Room
Gertrude Bunt
University of Goettingen, Germany
Visualization of multiple coupled signaling events in a single cell by fluorescence
Chair: G. Scarcelli lifetime pattern analysis.
Chair: R. Bizzarri
10.00 - 10.45 Optical application to biomedicine
Advanced imaging techniques I
Chair: G.Scarcelli
Chair: R. Bizzarri
Andrea Curatolo
University of Western Australia, Australia
Portable 3D optical coherence elastography for applying microscopic imaging of
tissue mechanical properties in the clinic.
Barbara Storti
NEST, Pisa Italy
Multiplexing correlation spectroscopy
and resonance energy transfer unveils dynamical interactions between transient
receptor potential vanilloid 1 (trpv1) and
George A. Stanciu
University Politehnica Bucharest, Romania microtubules at nanoscale in living cells.
Electrical charge on the hydroxyapatite Dominika O. Trembecka-Lucas
surface investigated by laser scanning Jagiellonian Universit. Poland
microscopy.
Interaction between HP1 dimer and
PCNA in DNA replication and repair, as
S. Beke
revealed by bimolecular fluorescence
Istituto Italiano di Tecnologia (IIT), Gencomplementation assay (BiFC), and
ova, Italy
Excimer laser-based stereolithography FRET/FLIM.
for rapid fabrication of rigid biodegrad- Simona Rodighiero
able photopolymer scaffolds for tissue Fondazione Filarete, Milan, Italy
engineering.
FRET study of 4.1R, ICln and actin interactions: a link between cell morphology
and cell proliferation.
Page 6 • Program • OWLS • Genoa, 4-6 July 201 2
10.45 - 11.15 Giuliano Scarcelli
Harvard Medical School, USA
Brillouin microscopy to study the
bio-mechanical properties of cornea and
lens.
Ranieri Bizzarri
National Research Council, Italy
Novel probes and strategies for high-resolution fluorescence imaging in living
cells.
Chair: D.Sampson
Chair: G.Bunt
11.15 - 11.45 Coffee break
11.45 - 12.15 Scirocco-Libeccio Room
Monika Ritsch-Marte
Medizinische Universität Innsbruck, Austria
Applications of spatial light modulators in biophotonics: from optical tweezers to
advanced microscopy.
Chair: F.Difato
Parallel Sessions
12.45 - 13.30 Scirocco - Libeccio Room
Ponente Room
Plasmonics
Advanced imaging techniques II
Chair: E. Di Fabrizio
Chair: A. Periasamy
Emanuela Esposito
Antonella Battisti
CNR-ICIB Napoli, Italy
NEST, Pisa, Italy
Lab-on-Fiber Technology: Nanophoton- Intracellular pH measurements made
ics Within Optical Fibers.
simple by genetically-encoded fluorescent probes and phasor approach to
Fang Liu
FLIM.
Tsinghua University, Beijing
Integrated Bio-Sensor based on Hybrid Ottavia Golfetto
Coupler with Surface Plasmon Polariton University of California, Irvine, California
and Dielectric Waveguide.
Laurdan fluorescence lifetime as a biosensor for membrane fluidity.
Ranjeet Kumar
Indian Institute of Technology New Delhi,
India
Clustering of Au-NPs near plasmon excitation and enhanced scattering based
in-vitro detection of Ehrlich ascites carcinoma cells.
Deo R. Singh
University of Wisconsin Milwaukee, USA
Quaternary structure of wild type and
mutated rhodopsin at room and body
temperature using spectral FRET.
13.30 - 15.30 Lunch, posters, exhibition, Job corner.
Company Presentations
14.00 - 15.00 Scirocco - Libeccio Room
Olga Levai
Leica Microsystems
Opening the gate to Super-Resolution.
Ponente Room
Maria Marosvölgyi
Till Photonics
Combining advanced fluorescence imaging techniques on the same living sample:
spinning disk confocal, TIRF, FRAP(PA)
and FRET.
OWLS • Genoa, 4-6 July 2012 • Program • Page 7
15.30 - 16.00 Scirocco - Libeccio Room
Anthony Watts Oxford University, UK
Realizing bio-imaging potential: Enhancing bacteriorhodopsin-mediated
molecular scale, wavelength-selective photoswitching.
Chair: A. Periasamy
Parallel Sessions
16.00 - 16.30 Scirocco - Libeccio Room
Ilaria Testa
Max Planck Institute Goettingen, Germany
RESOLFT Nanoscopy in Life Sciences:
unraveling the mysteries with low light
levels
Ponente Room
Arthur Chiou
National Yang-Ming University, Taiwan
(R.O.C.)
Optical Tweezers Based BioMicrorheology
Chair: G. Vicidomini
16.30 - 17.15 Single molecule and super-resolution Optical Tweezers
Chair: I.Testa
Chair: D. Cojoc
Chair: A. Chiou
Paolo Annibale
Francesco Difato
EPFL, Lausanne, Switzerland
Istituto Italiano di Tecnologia, Genova,
Towards accurate dual color PALM im- Italy
aging.
Position and Force-clamp interferometric
tracking of an optically trapped probe to
Francesca Cella Zanacchi
study cytoskeleton dynamics of develIstituto Italiano di Tecnologia, Genova,
oping mouse neuron.
Italy
Super-resolution microscopy of large Pasquale Bianco
biological samples within a light sheet Università degli Studi di Firenze, Italy
based architecture.
The energy landscape of DNA overstretching transition determined with a
Davide Mazza
Dual Laser Optical Tweezers under force
Fondazione S.Raffaele, Milano, Italy
Measurement of binding in living cells by clamp.
single molecule tracking. A benchmark Dan Cojoc
for protein/scaffold interactions.
Materials Technology Institute, Trieste,
Italy
Local delivery of small numbers of attractant and repulsive guidance molecules to
neuronal cells by optical manipulation
techniques.
17.15 - 17.45 Mike Heilemann
Maria Luisa Calvo Padilla
Wuerzburg University, Germany
Universidad Complutense de Madrid, Spain
Quantitative Single Molecule Biology Holography and its applications.
with Photoswitchable Fluorophores.
Chair: A.Chiou
Chair: I.Testa
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17:45 - 18:15 Coffee break
18:15 - 18:45 Scirocco - Libeccio Room
Brad Amos Cambridge, UK
A laser-scanning microscope for optical sectioning of large-volume specimens such
as mouse embryos using a Mesolens.
Chair: C.J.R. Sheppard
Parallel Sessions
18.45 - 19.30 Scirocco - Libeccio Room
Ponente Room
Optical tomography
Optical application to biomedicine II
Chair: C.J.R. Sheppard
Chair: K. Braeckmans
Stephen P Morgan
University Park, Nottingham, UK
Pulsed ultrasound modulated optical tomography of tissue
Maria Pogorelova
Moscow State University, Moscow, Russian
Federation
Optical microtomography of early mouse
embryo with laser scanning microscopy
Ludovico Silvestri
University of Florence, Italy
Whole-brain neuroanatomy on the micron-scale with confocal light sheet microscopy
19.30-20.00 Martin J. Booth
Oxford University, UK
Adaptive optics for high resolution biological microscopy
Wei Zhang
Tsinghua University, Beijing, China
Trace level multi-gas sensing in mid-infrared hollow-core Bragg fiber supporting
multi-wavelength transmission
Tadelech
Laser-Induced Breakdown and X-Ray
Fluorescence Spectroscopic Techniques
in Detection of Elements in Tea Leaves
Tewfik Soulimane
University of Limerick, Ireland
Photosterilisable Textiles for Improved
Hospital Hygiene
Dayong Jin
Macquarie University, Sydney, NSW 2109,
Australia
Novel coding and decoding in suspension
Chair: C.J.R. Sheppard arrays for accelerated biomolecular discovery and personalized medicine.
Shan Shan Kou
EPFL, Lausanne, CH-1015 Switzerland
Three-dimensional (3D) Monte Carlo
Simulation of Born’s Diffractions for Cellular Analysis
OWLS • Genoa, 4-6 July 2012 • Program • Page 9
Thursday, July 5th
8.30 - 9.00 Scirocco - Libeccio Room
RMS Lecture
Tony Wilson Oxford University, UK
Making Light Work in Microscopy.
Chair: F. Brakenhoff
Parallel Sessions
9.00 - 9.30 Scirocco - Libeccio Room
Martin Oheim
Université Paris Descartes, France
Non-evanescent excitation light in objective-type TIRF microscopy limits to
quantitative imaging and super-resolution microscopy.
Ponente Room
Amitabha Chattopadhyay
Centre for Cellular and Molecular Biology
Hyderabad, India
Organization and Dynamics of the Serotonin-1A Receptor in Live Cells using
Fluorescence Microscopy.
Chair: J. Bewersdorf
9.30 - 10.15 STED microscopy I
Chair: S. Maiti
Advanced Imaging Techniques III
Chair: J. Bewersdorf
Chair: A. Chattopadhyay
Giuseppe Vicidomini
Istituto Italiano di Tecnologia, Genoa, Italy
Towards Low-Intensity STED Microscopy
by Time Gating.
Lorena Benedetti
University of Milan, Italy
Morphological features of 4.1R overexpressing cells characterized with Correlative Light-Scanning Electron Microscopy
and observed with gated-STED nanoscopy.
Cosimo Arnesano
University of California Irvine, Irvine, California
Digital Parallel Frequency-Domain Spectroscopy for Tissue Imaging.
Stefano Coppola
Sapienza - University of Rome, Italy
Intracellular trafficking of multicomponent lipoplexes: a fluorescence microscopy study.
Tilman Rosales
National Institutes of Health, USA
Instrumentation for super-resolution using new low power probes.
10.15 - 10.45 Joerg Bewersdorf
Yale University, USA
New Approaches in STED Microscopy:
TIRF and Phase Retrievaly.
Anna Letizia Allegra Mascaro
University of Florence, Italy
In vivo imaging of climbing fibers reactive
plasticity after laser axotomy.
Sudipta Maiti
Tata Institute of Fundamental Research,
India
Protein misfolding versus aggregation:
Chair: M. Oheim following the early events with an ultrasensitive fluorescence tool.
Chair: A. Chattopadhyay
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OWLS • Genoa, 4-6 July 2012 • Program • Page 10
10:45 - 11:15 Coffee break
11.15 - 11.45 Scirocco - Libeccio Room
Ernst H.K. Stelzer
Goethe University Frankfurt , Germany
From the ultramikroskop to theta microscopy to tetrahedral microscopy to light
sheet-based fluorescence microscopy.
Chair: F. Cella Zanacchi
Parallel Sessions
11.45 - 12.30 Scirocco - Libeccio Room
Ponente Room
Single Particle Tracking
Label Free Techniques
Chair: Z. Lavagnino
Chair: P. O’Shea
Hendrik Deschout
Ghent University, Belgium
On-chip sheet illumination for single-particle tracking measurements in
bio-fluids.
Katrien Forier
Ghent University, Belgium
Single particle tracking microscopy: Towards nanomedicines for the treatment
of chronic pulmonary infections in cystic
fibrosis patients.
Christelle Rosazza
University of Konstanz, Germany
Single Particle Tracking of DNA in cells
delivered by electroporation.
12.30 - 13.00 Kevin Braeckmans
University of Ghent, Belgium
Sizing Nanomatter in Biological Fluids by
Fluorescence Single Particle Tracking.
Francesca Romana Bertani
National Research Council, Rome, Italy
Hyperspectroscopic confocal reflectance
imaging of cultured cells.
Alessandro Candiani
University of Parma, Italy
Label-free DNA sensor based on optical
fiber grating devices.
Dan Cojoc
National Research Council, Trieste, Italy
Secondary speckle sensing microscopy
for fast detection of malaria.
Paul O’ Shea
University of Nottingham, UK
Imaging specific proteins in cells;
time-resolved dynamics and disposition
Chair: Z. Lavagnino without GFP.
Chair: A. Chattopadhyay
13.00 - 15.00 Lunch, posters, exhibition, Job corner.
Company Presentations
14.00-15.00 Scirocco-Libeccio Room
Ponente Room
Luciano Lucas
Alessandro Cometta
Bitplane
Zeiss
Imaris: the 3D/4D image analysis soft- A super-resolution close-up view: from
ware
SIM to PALM
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OWLS • Genoa, 4-6 July 2012 • Program • Page 11
15.00 - 15.30 Scirocco-Libeccio Room
Colin Sheppard
National University of SIngapore, Singapore and Istituto Italiano di Tecnologia, Italy
Imaging in the phase contrast microscope.
Chair: P.Saggau
15.30 - 16.00 Gail McConnell
University of Strathclyde, UK
Promising new wavelengths for multiphoton laser scanning microscopy: thinking
outside the Ti:Sapphire
Chair: P.Saggau
Parallel Sessions
16.00 - 17.30 Scirocco - Libeccio Room
Ponente Room
Non Linear/Multiphoton Microscopy Fluorescent Probes/Sensors
Chair: G. McConnell
Chair: F. Brandi
Giulia Borile
Komal Bagga
University of Padova, Italy
Istituto Italiano di Tecnologia, Genoa, Italy
Multiphoton fluorescence Ca2+ imaging Laser-based generation of luminescent
in acute cardiac slices.
bio-nanomarkers for cell imaging.
Albrecht Haase
Ilaria De Santo
University of Trento,Italy
Istituto Italiano di Tecnologia, Naples, Italy
In-vivo morpho-functional imaging of Understanding Drug Localization in Doxthe honeybee brain .
orubicin Loaded TiO2 Nanotube Arrays
through Fluorescence Correlation SpecB. Leshem
troscopy analysis.
Paris Descartes University
Temporally focused illumination is inher- Joanna L Richens
ently robust against scattering.
University of Nottingham, UK
Plasmonic-Based Biosensors in Disease
Leonardo Sacconi
National Research Council, Florence, Italy Diagnosis.
Optical recording of intracellular action Shu-Han Wu
potential propagation in cardiac myo- National Yang-Ming University, Taipei,
cytes.
Taiwan
Dynamic Study of Cell Secretion using
Godofredo Bautista
Microfluidic Traps and Gold Nanoslits
Tampere University of Technology, FinBased Surface Plasmon Sensors.
land
Wide field third-harmonic generation Milena Stephan
microscopy of turbid media.
Georg-August University of Goettingen,
Germany
Neeven A. Hosny
Combining Fluorescence Microscopy
Imperial College London, Great Britain
Viscosity imaging of lipid-based micro- with Reflectometry: Development of a
bubbles using fluorescence lifetime im- Biomembrane Sensor.
aging (FLIM) of molecular rotors.
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OWLS • Genoa, 4-6 July 2012 • Program • Page 12
17.30 - 18.00 Scirocco - Libeccio Room
Francesco S. Pavone
European Laboratory for Non Linear Spectroscopy, University of Florence
In Vivo Reactive Neural Plasticity Investigation by means of Correlative Light-Electron Microscopy.
Chair: P. Bianchini
18.00 - 19.15 OWLS Assembly with Coffee break
20.30 Social Dinner
Villa dello Zerbino
http://www.villalozerbino.it/
OWLS • Genoa, 4-6 July 2012 • Program • Page 13
Friday, July 6th
9.00 - 9.30 Scirocco - Libeccio Room
Enrico Gratton
University of California Irvine, US
Super-resolution by feedback imaging: mechanisms of translocation through the
nuclear pore complex.
Chair: P. Bianchini
9.30 - 10.00 Daniel Farkas
Cedars-Sinai Medical Center, USA
Multimode Optical Molecular Imaging of Disease for Better Diagnosis and
Treatment.
Chair: P. Bianchini
Parallel Sessions
10.00 - 10.45 Scirocco - Libeccio Room
Ponente Room
Amyloid Studies
Optics in the environmental science
Chair: C. Canale
Chair: F. Baldini
Valeria Musi
Ecole Polytechnique Fédérale de Lausanne,
Switzerland
Bloch surface wave optical platform for
sensing the aggregation kinetics of amyloid beta peptides. Towards the early detection of Alzheimer’s disease.
Malgorzata Biczysko
Istituto Italiano di Tecnologia, Pisa, Italy
Simulation of optical spectra line-shapes
for biomolecules in natural environmental conditions.
Niels Zijlstra
University of Twente, Netherlands
Establishing the composition of alpha-synuclein amyloid oligomers using
single-molecule photobleaching.
Dayong Jin
Macquarie University, Sydney, Australia
Tuning the Rare-earth Nanoparticles for
Background-free Biosensing.
Sanjaya Thilakarathne
Saitama University, Japan
Bidyut Sarkar
Ultra short term growth dynamics of
Tata Institute of Fundamental Research,
Soybean and Radish under ozone stress
Mumbai, India
using Statistical Interferometry TechLabel free dopamine imaging with multi- nique - a comparison with conventional
photon ultraviolet microscopy.
ozone assessment measures.
10.45 - 11.15 Coffee break
11.15 - 11.45 Scirocco - Libeccio Room
Nicolai Urban
Max Planck Institute Goettingen, Germany
Dynamic superresolution microscopy deep inside living brain tissue.
Chair: P. Bianchini
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Parallel Sessions
11.45 - 12.30 Scirocco - Libeccio Room
Ponente Room
Sted Microscopy II
Novel Techniques
Chair: B. Harke
Chair: E. Gratton
Paolo Bianchini
Cristophe Silien
Istituto Italiano di Tecnologia, Genoa, Italy University of Limerick, England
Single wavelength 2PE-STED nanoscopy. Chemical Imaging with Super Resolution
Infra-Red Absorption.
Brian R. Rankin
Mobius Photonics, CA, USA
A new laser for STED
P. Nandakumar
BITS Pilani K. K. Birla Goa Campus, India
Laser scanning photothermal microscopy.
Jay R. Knutson
National Institutes of Health, USA
William M. Whelan
A new class of low-power probes for mul- Atlantic Veterinary College, Canada
ticolor nanoscopy.
Frequency spectrum analysis in photoacoustic tissue characterization.
12.30 - 13.00 Peter Saggau
Ari T. Friberg
Baylor College, Texas, USA
National Institute of Advanced Industrial
Advanced Optical Imaging for Re- Science and Technology (AIST), Japan
verse-Engineering the Brain
Classical Ghost Imaging of Phase Objects
and through Phase Aberrations
Chair: B. Harke
Martijn Stopel
University of Twente, Netherlands
Room temperature excitation spectroscopy of single quantum dots
13.00 - 15.00 Lunch, posters, exhibition, Job corner.
15.00 - 15.30 Scirocco - Libeccio Room
Ponente Room
Maria Garcia Parajo
ICFO- Institut de Ciences Fotonique Barcelona, Spain
Cell surface nanoscopy using single molecule optical nanotools
Chair: M. Heilemann
15.30 - 16.00
EBSA Lecture
Andreas Zumbusch
Universitat Konstanz, Germany
Coherent anti-Stokes Raman Scattering microscopy of intracellular dynamics
Chair: M. Heilemann
OWLS • Genoa, 4-6 July 2012 • Program • Page 15
Parallel Sessions
16.00 - 17.30 Scirocco - Libeccio Room
Nanoparticles & Smart Materials
Ponente Room
Force Microscopy
Chair: M. Lorenzoni
Chair: C. Canale
Athanassia Athanassiou
Istituto Italiano di Tecnologia, Genoa, Italy
Nanocomposite surfaces with photoinduced wettability gradient for directional
growth of cells.
Marco Capitanio
European Laboratory for Non-linear Spectroscopy, Sesto Fiorentino, Italy
Ultrafast force-clamp spectroscopy of
single biomolecules
Francesco Baldini
National Research Council, Floresnce, Italy
PMMA-nanoparticles for molecular beacon delivery in cells for survivin mRNA
detection.
Josè L.Toca-Herrera
University of Natural resources and Life
Sciences Wien, Austria
Delivering mechanical and interfacial
sample properties by combining optical
and atomic force microcopies
Despina Fragouli
Istituto Italiano di Tecnologia, Lecce, Italy
Rendering cellulose sheets antibacterial
using silver nanoparticles formed by a
laser ablation technique.
Jenu Varghese Chacko
Istituto Italiano di Tecnologia, Genoa, Italy
AFM STED: Targeted probing with high
spatial precision
Alemu Kebede
Addis Ababa University, Etiopia
Controlled Synthesis, Characterization
and Application of Iron Oxide Nanoparticles for Oral Delivery of Insulin.
Raman Spectroscopy
Laura D’Alfonso
Università degli Studi di Milano Bicocca,
Milan, Italy
Intracellular diffusion of Gold Branched
Nanoparticles.
Italy
Modulated Raman spectroscopy: a
promising biophotonics tool for early
cancer diagnosis
Chair: R. Proietti
Riccardo Cicchi
European Laboratory for Non-linear Spectroscopy, Sesto Fiorentino, Italy
Enrico Rampazzo
A combined fluorescence-Raman specUniversità di Bologna, Italy
troscopic probe for the diagnosis of
Fluorescent Silica-Core/PEG-Shell Nano- melanocytic lesions
particles with Tunable emission for Imag- Anna Chiara De Luca
ing and Labeling.
Institute of Protein Biochemistry, Naples,
Page 16 • Program • OWLS • Genoa, 4-6 July 201 2
Nicholas Smith
Osaka University, Japan
Raman imaging and analysis: from quantification of cellular morphology to RNA
structure and intramolecular interactions.
17.30 - 18.00
Closing Ceremony
Scirocco-Libeccio Room
OWLS 2012 Closing Lecture
Satoshi Kawata
Osaka University, Japan
Plasmons, Raman, nano and 3D, all in together.
Chair: A. Diaspro
18.00 - 18.30
Poster prizes
Sponsored by OWLS, Université Laval Québec, Camera di Commercio
di Genova
Farewell with Focaccia and white wine
Sponsored by Camera di Commercio, Leica Microsystems, Bitplane
Friday, July 6 2012 8.30pm
City Lights by LAMBS
www.lambs.it
Piazza De Ferrari, Genova (De Ferrari Square)
Saturday, July 7 2012 Full Day
Cinque Terre Tour
www.cinqueterre.it
OWLS • Genoa, 4-6 July 2012 • Program • Page 17
OWLS 2012
Poster Session
“An insight of NoBoDys PerFect” Exhibition
P1 - P6
Page
P1 Gaser N. Abdelrasoul1,2 , Raffaella Magrassi3, Marco Scotto d’Abbusco1, Alberto 120
Diaspro1, 2
1 Department of Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, 16163 Genova, Italy
2 Department of Physics University of Genova, via Balbi 5, 16126 Genova, Italy
3 National Research Council (CNR) - Institute of Marine Sciences (ISMAR), Genova, Italy
2PE Imaging of Cancer Cells Using PEGylated Gold Nanorods as Biocompatible Contrast Agent.
P2 Giulio Caracciolo1, Daniela Pozzi1, Anna Laura Capriotti2, Chiara Cavaliere2,
Francesco Cardarelli3, Angelo Bifone3, Giuseppe Bardi3, Fabrizio Salomone3 and
Aldo Laganà2
123
1 Department of Molecular Medicine, ‘Sapienza’ University of Rome, Viale Regina Elena, 324, 00161,
Rome, Italy
2 Department of Chemistry, ‘Sapienza’ University of Rome, P.le A. Moro 5, 00185, Rome, Italy,
3 Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro
12, 56127 Pisa, Italy
Protein corona controls the uptake delivery mechanisms of lipid gene vectors in cancer cell
P3 Tatjana Dramićanin, Lea Lenhardt, Ivana Zeković, Nasta Tanić and Miroslav D.
Dramićanin
128
Vinča Institute of Nuclear Sciences, University of Belgrade, P.O. Box 522, 11001 Belgrade, Serbia
Breast Cancer Detection by Support Vector Machine Classification of Synchronous
Fluorescence Spectroscopy Data
P4 Ivana Zekovića, Lea Lehnardta*, Tatjana Dramićanina, Miroslav D. Dramićanina
a Vinča Institute of Nuclear Sciences, University of Belgrade, PO Box 522, 11001 Belgrade, Serbia
Application of Supervised Self-Organizing Maps in Skin Melanoma Diagnosis by Total
Synchronous Fluorescence Spectroscopy
P5 Elisabetta M. Caspani, Philip H. Crossley, Carolina Redondo-Garcia, Salvador
Martinez
144
*
Laboratory of Experimental Embryology, Instituto de Neurociencias UMH-CSIC, Alicante, Spain
Malignopodium: a new player in glioma progression
P6 Roberto Cighettia, Matteo Piazzaa, Valentina Calabresea, Gaetana Damorea
and Francesco Peria
a Università degli Studi di Milano-Bicocca, Piazza della Scienza, 2 – 20126 – Milano, Italy
New fluorescent molecular tools to investigate LPS recognition
Page 18 • Program • OWLS • Genoa, 4-6 July 201 2
124
P16 - P30
P16 Hanna Beckera,2, Amy Gladfelter3, Rudolf Oldenbourg1
Page
121
1 Marine Biological Laboratory, Woods Hole, Massachusetts, USA
2 Universität Konstanz, Konstanz, Germany
3 Dartmouth College, Hanover, New Hampshire, USA
Quantitative and high resolution imaging of fluorescence anisotropy is shedding
light on septin architecture in the yeast bud neck
P17 Iván, Coto Hernández1,2, Giuseppe, Vicidomini1, Alberto, Diaspro1,2
125
1 Istituto Italiano di Tecnologia, Genova, Italia
2 Universita Degli Studi di Genova, Genova, Italia
Generation Of Super Continuum Source And Its Application In Nanoscopy
P18 William F. Falco, Joelson Fernandes, Evaristo A. Falcão, Anderson R.L. Caires
130
Grupo de Óptica Aplicada, Universidade Federal da Grande Dourados, Dourados, MS, Brazil
Effects on Photosynthetic Activity of Plants Induced by Silver Nanoparticles: Monitoring by Chlorophyll Fluorescence
P19 Joelson Fernandes1, William F. Falco1, Samuel L. Oliveira2, Anderson R.L.
Caires1
131
1 Grupo de Óptica Aplicada, Universidade Federal da Grande Dourados, Dourados, MS, Brazil
2 Grupo de Óptica e Fotônica, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS,
Brazil
Changes on Photosynthetic Activity of Herbicide-tolerant Soybean Plants Induced
by Glyphosate
P20 Jenni Karttunen1, Sari Mäntynen1, Heli Lehtivuori1, Janne Ihalainen1, Teemu
Ihalainen1, Jaana K. H. Bamford1 and Hanna M. Oksanen2
138
1 Department of Biological and Environmental Science and Nanoscience Center, PO Box 35, 40014
University of Jyväskylä, Jyväskylä, Finland
2 Institute of Biotechnology and Department of Biosciences, PO Box 56, 00014 University of Helsinki, Helsinki, Finland
Protein Localization and Interactions in Bacteria Studied with Confocal Microscopy
and Fluorescence Lifetime
P21 Volkan KILIÇ, Gözde AYDOĞAN KILIÇ, Ahmet ÖZATA
140
Anadolu University, Faculty of Science, Department of Biology, 26470, Eskişehir, TURKEY
Light microscopic pathology in Common carp ( Cyprinus carpio L.,1758) from Sakarya River (TURKEY)
P22 Lea Lenhardt1*, Ivana Zeković1, Tatjana Dramićanin1, Živoslav Tešić2, Dušanka
Milojković-Opsenica2, Miroslav D. Dramićanin1
143
1 Vinča Institute of Nuclear Sciences, University of Belgrade, PO Box 522, 11001 Belgrade, Serbia
2 Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
Assessment of Fluorescence and Infrared Spectroscopy Capabilities for the Determination of Botanical Origin of Serbian Unifloral Honey
OWLS • Genoa, 4-6 July 2012 • Program • Page 19
P16 - P30
P23 Daniela Pozzi1, Giulio Caracciolo1, Francesco Cardarelli2, Angelo Bifone2, Heinz
Amenitsch3
Page
152
1 Department of Molecular Medicine, “Sapienza” University of Rome, Viale Regina Elena, 324,
00161, Rome, Italy
2 Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro
12, 56127 Pisa, Italy
3 Institute of Biophysics and Nanosystems Research, Austrian Academy of Sciences, Schmiedelstrasse 6, A-8042 Graz, Austria
Confocal microscopy makes visible cholesterol-mediated gene delivery mechanisms
P24 Liliia Rohoza, Tatyana Dyubko, Sergey Galchenko, Boris Sandomirsky
Institute for Problems of Cryobiology and Cryomedicine fo the NAS of Ukraine, Kharkiv
Spectrofluorimetric investigation of interactions of heart peptides with blood serum
albumin
P25 Sebastian Tannert1, Peter Kapusta1, Alexander Glatz1, Christian Litwinski1, Uwe
Ortmann1, Rainer Erdmann1, Katrin Hoffmann2, Ute Resch-Genger2
153
154
1 PicoQuant GmbH, Rudower Chaussee 29, 12489 Berlin, Germany, www.picoquant.com
2 Federal Institute for Materials Research and Testing (BAM), Richard-Willstätter-Str. 11, 12489
Berlin, Germany)
On the spectral sensitivity calibration of fluorescence spectrometers: Extension to
the NIR, polarization and grating effects
P26 Anatoliy Tatarets1*, Yevgeniy Povrozin1, Olena Obukhova1, Iryna Fedyunyayeva1, Ewald Terpetschnig2, Leonid Patsenker1,2
155
1 State Scientific Institution “Institute for Single Crystals”, NAS of Ukraine, 60 Lenin Ave., 61001
Kharkiv (Ukraine)
2 SETA BioMedicals, LLC, 2014 Silver Ct East, Urbana, 61801, IL, USA,
Novel Squaraine Probes for Proteins and Fluorescence Imaging Applications
P27 Francesco Urciuolo1, Giorgia Imparato1, Costantino Casale1, Fabio Formiggini1,
Paolo Netti1,2
1 Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci n, 53 80125 Napoli
2 Interdisciplinary Research Centre on Biomaterials (CRIB), University of Naples Federico II, P. Le
Tecchio 80, 80125 Naples, Italy
Coupling Engineered Living Tissue with Multiphoton Tomography : towards in vitro
understating of biological phenomena
Page 20 • Program • OWLS • Genoa, 4-6 July 201 2
156
P31 - P45
P31 William Dallari, Marco Allione, Elena Samoylova, Marco Scotto d’Abbusco,
Alberto Diaspro
Page
126
Nanophysics, Istituto Italiano di Tecnologia, via Morego, 30, 16163 Genova
Permanent and rewritable 3D optical data storage through multi-photon absorption
P32 Silvia Galiani1, Benjamin Harke1, Giuseppe Vicidomini1, Gabriele Lignani2, Fabio
Benfenati2, Hanako Tsushima2, Evelina Chieregatti2, Paolo Bianchini1, Alberto
Diaspro1
133
1Nanophysics, Istituto Italiano di Tecnologia, Genoa, Italy
2Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Genoa, Italy
Optimization of a STED nanoscope
P33 Manuel Gunkela, Nina Beila, Jürgen Benekea, Slawek Mazura, Jürgen Reymanna, Bastian Schumachera and Holger Erflea
135
a Viroquant-CellNetworks RNAi Screening Facility, BioQuant, University of Heidelberg, Germany
Automated microscopy in RNAi screening experiments
P34 Timo Hakkarainen1, Tero Setälä1, Ari T. Friberg1,2,3
136
Near-Field Imaging of Interacting Nano-Particles
P35 Christian Jüngst*and Andreas Zumbusch
137
1 Department of Applied Physics, Aalto University, Box 13500, FI-00076 Aalto, Finland
2 Department of Physics and Mathematics, University of Eastern Finland, Box 111, FI-80101 Joensuu,
Finland
3 Department of Microelectronics and Applied Physics, Royal Institute of Technology (KTH), Electrum 229, SE-164 40 Kista, Sweden
Department of Chemistry, University of Konstanz, 78457 Konstanz, Germany
Label-free imaging of Lipid Droplets in living cells
P36 Marcelle Koenig1, Steffen Ruettinger1, Sebastian Tannert1, Thomas Schoenau1,
Christian Litwinski1, Olaf Schulz2, Michael Wahl1, Marcus Sackrow1, Kristian
Lauritsen1, Felix Koberling1, Robert Ros2, and Rainer Erdmann1
141
1 PicoQuant GmbH, Rudower Chaussee 29, 12489 Berlin, Germany, [email protected]
2 Arizona State University, Lab Robert Ros, Physics Department, Tempe, AZ 85287-1504, USA
Pushing the boundaries of single molecule detection
P37 Zeno Lavagninoa1, Francesca Cella Zanacchi1, Alberto Diaspro1,2
142
Thick Sample imaging and scattering effects: a phantom sample based characterization of Single Plane Illumination Microscopy performances
P38 Aimar Namma2, Andres Arend1, Marina Aunapuua,2
146
1 Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30,16163 Genova, Italia
2 LAMBS- Dept. of Physics – University of Genoa, Via Dodecaneso 33, 16153 Genova, Italia
1 Department of Anatomy, University of Tartu, Ravila 19, 50411 Tartu, Estonia
2 Department of Veterinary Medicine, Estonian University of Life Sciences, Fr Kreutzwaldi 62, 51014
Tartu
Computer assisted image analysis in the detection of expression of bone morphogenetic proteins in the neural tube of human embryos
P39 Marco Zanella1†, Luca Ceseracciu2†, Roberta Ruffilli1, Alberto Diaspro2, Liberato Manna1
158
1 Nanochemistry, Istituto Italiano di Tecnologia, via Morego 30, 16163 Genova, Italy
2 Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, 16163 Genova, Italy
Polarized plasmonic absorption in micrometer long copper(I) selenide nanowires
OWLS • Genoa, 4-6 July 2012 • Program • Page 21
P46 - P60
P46 Abebe Belay
Adama Science and Technology University, School of Natural Sciences, Department of Physics,
P.O.Box, 1888, Adama, Ethiopia,
Page
122
The Hetero-Association of Caffeine (CA) with 5-Caffeoyl quinic Acid (5-CQA) and
Ethidium Bromide (EB) in Aqueous Solution
P47 Mariagrazia Di Luca1*, Francesco Cardarelli2, Ranieri Bizzarri 1,3
127
Fluorescent recombinant virus like particles as nano-carriers of sensors
P48 Sabato Fusco, Valeria Panzetta, Paolo Antonio Netti
132
1 NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, Piazza San Silvestro 12 - 56127
Pisa
2 Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro
12, 56127 Pisa
3 Istituto di Biofisica - CNR, via Moruzzi 1, 56124 Pisa
Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia Largo Barsanti e Matteucci, 53 80125 Napoli
Cell Mechanical Characterization by Particle Tracking Microrheology in Optical Microscopy
P49 Gallus Lorenzo1*, Ferrando Sara1, Gambardella Chiara1, Amaroli Andrea1,
Faimali Marco2, Veronica Piazza2, Bianchini Paolo3, Masini Maria Angela1
134
1 Dip. di Scienze della Terra, dell’Ambiente e della Vita (DiSTAV), Univ. di Genova
2 Ist. di Scienze Marine, ICMM, CNR, Area di Genova
3 Dipartimento di Nanofisica, Istituto Italiano di Tecnologia Genova
Aquaporins in cyprid of barnacle Balanus amphitrite (=Amphibalanus amphitrite)
(Cirripedia, Crustacea)
P50 Gözde Aydoağan KILIÇ, Volkan KILIÇ, Ahmet ÖZATA
Anadolu University, Faculty of Science, Department of Biology, 26470, Eskişehir, TURKEY
Autometallographic localization of heavy metals and immunoperoxidase detection
of metallothionein induction in Common carp (Cyprinus carpio L.1758) from Sakarya
River (TURKEY)
P51 Keurison F. Magalhães1,3, Anderson R.L. Caires1*, Margarete S. Silva2, Samuel L.
Oliveira3
139
145
1 Grupo de Óptica Aplicada, Universidade Federal da Grande Dourados, Dourados, MS, Brazil
2 Grupo de Estudo e Desenvolvimento de Materiais, Universidade Estadual de Mato Grosso do Sul,
Dourados, MS,Brazil
3 Grupo de Óptica e Fotônica, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS,
Brazil
The Role of Fatty Acid Methyl Esters on Optical Properties of Biodiesel
P52 Olena Obukhova1*, Anatoliy Tatarets1, Olga Kolosova1, Iryna Fedyunyayeva1,
Leonid Patsenker1,2, Ewald Terpetschnig2
1 State Scientific Institution “Institute for Single Crystals”, NAS of Ukraine, 60 Lenin Ave., 61001
Kharkiv (Ukraine)
2 SETA BioMedicals, LLC, 2014 Silver Ct East, Urbana, 61801, IL, USA
Highly Photostable Biomedical Markers Based on Thiosquarylium Dyes
Page 22 • Program • OWLS • Genoa, 4-6 July 201 2
147
P53 Silvio Panettieri1 Giovanni Signore2 Ranieri Bizzarri2
148
1 NEST, Istituto Nanoscienze - CNR, Piazza S. Silvestro 12, 56127 Pisa
2 Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro
12, 56127 Pisa
Synthesis, stereochemical characterization, and photophysical features of an innovative 3-styryl coumarin acting as polarity/viscosity sensor for bioanalytical use
P54 Leonid Patsenker1,2*, Ewald Terpetschnig2, Anatoliy Tatarets1, Olena Obukhova1, Olga Kolosova1, Larysa Markova1, Inna Yermolenko1
149
1 State Scientific Institution “Institute for Single Crystals”, NAS of Ukraine, 60 Lenin Ave., 61001
Kharkiv (Ukraine).
2 SETA BioMedicals, LLC, 2014 Silver Ct East, Urbana, 61801, IL, USA,
Next Generation Fluorescent Dyes for Biomedical Applications
P55 Alessia Petrelli, Claudio Canale, Alberto Diaspro and Silvia Dante
Nanophysics Department, Fondazione Istituto Italiano di Tecnologia, Genova (Italy)
Quantitative evaluation of cell adhesion on patterned surfaces
P56 Ferruccio Pisanello1,2*, Luigi Martiradonna1, Godefroy Leménager3, Alberto
Bramati3, Massimo De Vittorio1,4
150
151
1 Center for Bio-­Molecular Nanotechnologies @UNILE, Istituto Italiano di Tecnologia, 73010 Arnesano (Lecce), Italy
2 Center for Neuroscience and Cognitive Systems @UNITN, Istituto Italiano di Tecnologia, 38068
Rovereto (TN), Italy
3 Laboratoire Kastler Brossel, Université Pierre et Marie Curie, CNRS UMR 8552, Ecole Normale
Supérieure, 75005 Paris, France
4 National Nanotechnology Laboratory, CNR-­‐Nano, Università del Salento, Dipartimento Ingegneria dell’innovazione, 73100 Lecce, Italy
Anisotropic colloidal nanocrystals as ultrastable polarized quantum emitters
P57 Kelly-Ann Vere, Joanna Richens and Paul O’Shea
Cell Biophysics Group, Institute of Biophysics, Imaging and Optical Science (IBIOS), School of Biology, University of Nottingham, UK NG7 2RD
157
Electrostatic mapping of molecular interactions on the cell surface; identification of
novel cell-surface receptor systems
OWLS • Genoa, 4-6 July 2012 • Program • Page 23
OWLS Member Assembly
July 5th, 2012 in Genoa, Italy (17:30-19:00)
Porto Antico Congress Center – Magazzini del Cotone – Scirocco-Libeccio Room
Agenda
Extra content: Lecture by Francesco Pavone (see program)
1. Discussion of the Agenda
2. President’s Report
3. Update of bylaws
4. Elections
❚❚Board
❙❙ President, Vice-President, Secretary, Treasurer
❙❙ OWLS Representative to ICO
❚❚Regional Council Representatives
❙❙ Africa, North America, South America, Asia, Australia/New Zealand, Europe
❚❚Standing Committees
❙❙ Bylaws and Regulatory Statutes;
❙❙ Technology Transfer, Standardization, and Technology Assessment;
❙❙ Finances;
❙❙ Education and Ethics
5. Meeting schedule
❚❚OWLS 13 Location
❚❚Co-Sponsoring
6. Treasurer’s report and decision of formal approval
❚❚Financial Report
7. New Members
8. Publications
9. Dues
10. Committee Reports
11. Miscellaneous
Prof. Alberto Diaspro
President of the International Society on Optics Within Life Sciences (OWLS)
Page 24 • Program • OWLS • Genoa, 4-6 July 201 2