The Effects of Gallic Acid on The Acute Necrotizing Pancreatitis in Rats

The Effects of Gallic Acid on The Acute
Necrotizing Pancreatitis in Rats
P-09.04.4-042
B. KURALI, D. US ALTAYII, C. KAHRAMANIII, E. ALHANIV, T. MAZLUM SENI, C. ERÇINV, N.
KÜÇÜK KENTVI, H. KARAI, S. KÖRI, A. ÖREMI
IDepartment of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University,
Trabzon, Turkey, IIUlubey Vocational School- Chemistry and Chemical Processing Technology
Department, Ordu University, Ordu, Turkey, IIISchool of Health, Düzce University, Düzce,
Turkey, IVDepartment of Surgery, Faculty of Medicine, Karadeniz Technical University,
Trabzon, Turkey, VDepartment of Pathology, Kocaeli University, Kocaeli, Turkey, VISchool of
Health, Gümüshane University, Gümüshane, Turkey
Reactive oxygen species, playing an active role in the early and late course of acute
pancreatitis, lead to dysfunction of cell membrane and releasing of lysosomal
enzymes, and thereby to the injury in pancreatic cells. Gallic acid, found in
vegetables such as green tea, is an active component which has antioxidant, antiinflammatory, antiviral, anticancer activities. The aim of this study was to
investigate the effects of gallic acid in experimental acute necrotizing pancreatitis
(ANP) model in rats. Eighteen male Sprague-Dawley rats were divided into three groups (6 rats in each
group). Group 1: sham + saline; group 2: ANP induced by intraductal glycodeoxycholic
acid and intravenous cerulein; and group 3: ANP + gallic acid (100 mg/kg/day,
intraperitoneal). At the end of 18th hours, pancreas histopathology was examined. The
levels of serum amylase as a diagnostic marker of pancreatitis, interleukin-6 (IL-6),
total antioxidant status (TAS), nitrite + nitrate, total thiols as antioxidant marker
and thiobarbituric acid reactive substances (TBARS) to measure malondialdehyde (lipid
peroxidation product) were determined by spectrophotometric methods.
Serum amylase, IL-6, plasma TBARS levels were significantly higher but total thiols
levels were lower than sham group in ANP group without treatment (p < 0.05). However;
TAS and nitrite + nitrate levels did not show any significant difference (p>0.05). On
the other hand, while serum amylase, IL-6 and TBARS levels were lower, total thiols
levels higher in gallic acid treatment group than in the untreated ANP group, but
statistically insignificant (p>0.05).
In conclusion, gallic acid treatment is beneficial but not sufficient to treat the
acute necrotizing pancreatitis in rats.