Biocem Sağlık Ürünleri San. ve Tic. Ltd. Şti.

Transkript

Biocem Sağlık Ürünleri San. ve Tic. Ltd. Şti.
Biocem Sağlık Ürünleri San. ve Tic. Ltd. Şti.
Hakkımızda
BioCem Ltd. Şti. Türkiye’de in-vitro diagnostik, araştırma ve tıbbi malzeme alanlarında hizmet
veren distribütör bir kuruluştur. Şirketimiz kusursuz hizmet anlayışı ve kendi alanında üstünlüğü
kanıtlanmış olan tedarikçileriyle sağlam temellere dayanan bir işbirliği içerisinde hizmet vermektedir.
Misyonumuz, değerli müşterilerimize sağlık ve hayat bilimi sektörünü destekleme hedefinde kusursuz kalite ve memnun edici servisi sunmaktır.
BioCem her biri alanında uzmanlaşmış kadrosuyla müşterileriyle yakın ilişki içerisinde kaliteyi sunmaktadır. Kurulduğu günden beri, araştırma çalışmalarına verdiği destek ve yatırım, müşteri
memnuniyetinin sağlanması ve zamanında teslimat ile sağlık pazarında saygın bir yer edinmiştir.
Genişleyen pazar payımızı artırabilmek adına, kararlı ve istikrarlı adımlarla çalışmaya devam
etmekteyiz. Saygın tedarikçi firmalarımızdan değerli müşterilerimize kadar olan bir zincir anlayışı
içerisinde, gelecekteki sayısız ortak başarıda birlikte çalışmak dileğiyle…
Temsilciliklerimiz
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Affymetrix Inc.
ExoSAP-IT
USB® ExoSAP-IT® PCR Product Cleanup means the ease of one-step purification.
The ExoSAP-IT method is a unique, one-tube, one-step, enzymatic cleanup reagent for PCR product cleanup.
• 100% sample recovery – no loss of PCR products regardless of the fragment size
• Removes excess primers and dNTPs – does not interfere with downstream applications
• Superior results with ExoSAP-IT reagent – improve accuracy with higher yields and full PCR product recovery
• Cited in over 7,000 publications – the enzymatic PCR cleanup solution used by leading scientists
USB ExoSAP-IT PCR Product Cleanup – available for standard and high-throughput, automated platforms
ExoSAP-IT PCR Product Cleanup
78250
20 Reactions
78200
100 Reactions
78201
500 Reactions
78202
2,000 Reactions
78205
5,000 Reactions
PCR products cleaned up with ExoSAP-­‐IT reagent prior to automated sequencing display superior sequencing results compared to the un-­‐cleaned equivalent www.biocem.com.tr
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Affymetrix Inc.
Biochemicals
USB biochemicals provide reliable, repeatable results. You demand dependable products, and we deliver the
highest-purity biochemicals and reagents with the proper documentation, every time. Top life science companies rely on
USB biochemicals to consistently meet their manufacturing requirements.
Acrylamides
Detergents
ACS Grade Biochemicals
Enzymes
Agaroses
General/Ultrapure Biochemicals
Albumins
Proteins
Antibiotics
Substrates
Bacterial Culture Media
Sugars
Buffers
Vitamins
Master Mixes & Kits
Markers
DNA Polymerases
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Affymetrix Inc.
VeriQuest
VeriQuest Fast SYBR Green qPCR Master Mix (2X)
A 2X pre-mixed formulation of SYBR Green qPCR Master Mix designed for fast mode cycling conditions
Part #
Description
Unit Size
75690 100 RXN
VeriQuest Fast SYBR Green qPCR Master Mix (2X)
1 ml for 100 reactions (20 µl)
75690 100 RXN
VeriQuest Fast SYBR Green qPCR Master Mix (2X)
5 ml for 500 reactions (20 µl)
75690 1000 RXN
VeriQuest Fast SYBR Green qPCR Master Mix (2X)
2 x 5 ml for 1,000 reactions (20 µl)
75690 2500 RXN
VeriQuest Fast SYBR Green qPCR Master Mix (2X)
5 x 5 ml for 2,500 reactions (20 µl)
75690 5000 RXN
VeriQuest Fast SYBR Green qPCR Master Mix (2X)
5 x 5 ml for 2,500 reactions (20 µl) VeriQuest SYBR Green qPCR Master Mix (2X)
Part #
Description
Unit Size
75600 40 RXN
VeriQuest Fast SYBR Green qPCR Master Mix (2X)
1 ml for 40 reactions (50 µl)
75600 200 RXN
VeriQuest Fast SYBR Green qPCR Master Mix (2X)
5 ml for 200 reactions (50 µl)
75600 400 RXN
VeriQuest Fast SYBR Green qPCR Master Mix (2X)
2 x 5 ml for 400 reactions (50 µl)
75600 1000 RXN
VeriQuest Fast SYBR Green qPCR Master Mix (2X)
5 x 5 ml for 1,000 reactions (50 µl)
75600 2000 RXN
VeriQuest Fast SYBR Green qPCR Master Mix (2X)
10 x 5 ml for 2,000 reactions (50 µl)
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Affymetrix Inc.
How to select the correct VeriQuest MM
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ARRAYS
C-Series
L-Series
Quantibody
G-Series
E-Series
Detection
Chemilluminescent
Solid Support
Membrane
Desingprinciple
Sandwich ELISA
Results
Semi-quantitative
Detection
Fluorescent or
Chemilluminescent
Solid Support
Glass Slide or Membrane
Desingprinciple
Direct Labelling (Biotin)
Results
Semi-quantitative
Detection
Fluorescent
Solid Support
Glass Slide
Desingprinciple
Sandwich ELISA
Results
Quantitative
Detection
Fluorescent
Solid Support
Glass Slide
Desingprinciple
Sandwich ELISA
Results
Semi-quantitative
Detection
Chemilluminescent
Solid Support
Membrane
Desingprinciple
Competitive ELISA
Results
Semi-quantitative
or Quantitative
Working sample volume: 1,000 µl (1 ml)
Advantages & Features:
No specialized equipment required
Easy to use
High specificity
Low background, high sample diversity, etc.
Working sample volume: 20-100 µl Advantages & Features;
Hundreds of molecules can be screened simultaneously, Lowest cost per target High specificity
Compatible with serum, plasma, and cell culture
media
Working sample volume: 50-100 µl Advantages & Features:
Lower sample consumption
Easy to use; high throughput
Wider dynamic range (up to 5 orders of magnitude)
Low background, high sample diversity, etc.
Working sample volume: 50-100 µl
Advantages & Features:
Lower sample consumption
Easy to use; high throughput
Wider dynamic range (up to 5 orders of magnitude)
Low background, high sample diversity, etc.
Working sample volume: inquire
Advantages & Features:
No specialized equipment required
Easy to use
High specificity
Low background, high sample diversity, etc.
CUSTUMIZE YOUR ARRAY
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ARRAYS
C-SERİES
CUSTUMIZE YOUR ARRAY
www.biocem.com.tr
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ARRAYS
QUANTİBODY: MULTİPLEX ELISA ARRAY
PROTEINS
Custom Recombinant Protein Service
Globulins
Adhesion Molecule/Cell Surface
Heat Shock Proteins
Adipokine/Obesity-related
Hormones and Steroids
Autoimmune/Allergy
Immunoglobulins
Bacterial/Fungal/Parasitic/Viral Antigens
Lipoproteins
Blood Coagulation Proteins
Miscellaneous
Cancer-related Markers
Molecular Biology
Cardiac Markers
Neuroscience
Collagen & Extracellular Matrix Proteins
Phosphorylation/Apoptosis/Signal Transduction/Regulation
Cytokines/Chemokines/Growth Factors/Receptors
Proteases & Other Enzymes
Cytoskeleton
Protein Kinases
Drug Derivative Proteins
CUSTUMIZE YOUR PROTEIN
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ELISA KITS
1 Plate Kit / 2 Plate Kit / 5 Plate Kit
1 Plate Kit / 2 Plate Kit / 5 Plate Kit
1 Plate Kit / 2 Plate Kit / 5 Plate Kit
1 Plate Kit / 2 Plate Kit / 5 Plate Kit
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ANTIBODYS
Adhesion molecule/Cell Surface
Adipokine/Obesity-related
Autoimmune/Allergy
Bacterial/Fungal/Parasitic/Viral Antigens
Blood Coagulation Proteins
Calcium Channel
Calcium signaling
Cancer-related Markers
Cardiac Markers
Cell Cycle
Collagen & Extracellular Matrix Proteins
Cytokines/Chemokines/Growth Factors/Receptors
Cytoskeleton
Drug/Derivative
Enzyme/Protease
Globulins
Glycoproteins
Heat Shock Proteins
Hormones and Steroids
Immunodetection Kit
Immunoglobulins
Lipoproteins
MHC Class
Miscellaneous
Neuroscience
NF-kB signaling pathway
Phosphorylation/Apoptosis/Signal Transduction/Regulation
Platelet and Homeostasis Proteins
Proteases & Other Enzymes
Protein Kinases
Stem Cell Markers
T-Cell Receptors
Tag
Transcription and its regulation
Transmembrane Protein and Receptor
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Hycult Biotech
ELISA & ANTIBODY
ELISA Specific ELISA for Innate Immunity, Cell & Tissue damage, Inflammation,
Disease-related Products
1x 96 det. and 2 x 96 det. ELISA Kits
Standard protocol of 3.5 hour
PRODUCT
MPO ELISA KIT (h,m,r) 1x96 det. / 2 x 96 det.
NGAL ELISA KIT (h) 1x96 det./ 2 x 96 det.
Calprotectin ELISA KIT (h) 1x96 det. / 2 x 96 det.
Pentraxin 3 ELISA KIT (h) 1x96 det. / 2 x 96 det.
Lactoferrin ELISA KIT (h) 1x96 det. / 2 x 96 det.
Nitrotyrosine ELISA KIT (h) 1x96 det. / 2 x 96 det.
H-FABP ELISA KIT (h,m,r) 1x96 det. / 2 x 96 det.
L-FABP ELISA KIT (h) 1x96 det. / 2 x 96 det.
I-FABP ELISA KIT (h) 1x96 det. / 2 x 96 det.
LAL Chromogenic Endpoint ASSAY 3 x 96 det
ANTI BODY Specific primary and secondary antibodies for FC,FS,F P,FI,IP,IA,W
The antibodies are arranged by species:
human, mouse and rat.
PRODUCT
CD14,Human,mAb 18D11
CD36 Human, mAb FA6-152
DAF, Human, mAb D17
Beta-Catenin, Human, mAb 9F2
Pentraxin 3, Human, mAb, MNB4
TLR4, Human, mAb3C3 biotinylated
JAM-A. Mouse, mAb, BV12
TLR4, Mouse, mAb Kat5-18
CD8,Rat, mAb 53-5D2
ZO-1,Human,pAb
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Primary Antibodys
BT-Laboratory Elisa Kits,
• Over 7400 different Elisa Kits
• For Human, Mouse, Rat, Rabbit, Bovine, Sheep, Cat, Horse, Chicken, Monkey, Fish, Goat, Canine
• Validated for Serum, blood plasma,Saliva, Urine sample types.
BT-Laboratory ELISA KITS
Human Tumor necrosis factor α,TNF-α ELISA KIT
Human Tumor necrosis factor α,TNF-α ELISA KIT
Human Interferon α,IFN-α ELISA Kit
Human Interleukin 1α,IL-1α ELISA Kit
Canine Interferon γ ,IFN-γ ELISA Kit
Canine Tissue factor,IF ELISA Kit
Bovine Anti-Thrombin Ⅲ,AT-Ⅲ ELISA Kit
Bovine malondialchehyche,MDA ELISA Kit
Bovine Endothelin 1,ET-1 ELISA Kit
Rat Mullerian Inhibiting Substance/Anti-Mullerian hormone,MIS/AMH ELISA Kit
Rat 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit
Mouse bradykinin,BK ELISA Kit
Mouse Insulin Like Growth Factor Binding Protein 3 (IGFBP3)ELISA KIT
Chicken Interleukin 1α,IL-1α ELISA Kit
Human myeloperoxidase,MPO ELISA Kit
Human Calf intestinal alkaline phosphatase,CIAP ELISA Kit
Human myeloperoxidase,MPO ELISA Kit
Human Thyroid-Peroxidase,TPO ELISA Kit
Human Matrix metalloproteinase 10,MMP-10 ELISA kit
Human Endothelial nitric oxide synthase,eNOS ELISA Kit
Human Super Oxidase Dimutase,SOD ELISA Kit
Human nitric oxide synthase,NOS ELISA Kit
Human Tumor necrosis factor soluble receptorⅡ,TNFsR-ⅡELISA KIT
Human soluble tumor necrosis factor 1,sTNF-1 ELISA Kit
Human reticulin IgG ELISA Kit
Human reticulin IgA ELISA Kit
Rat Interleukin 1,IL-1 ELISA Kit
Rat Interleukin 1α ,IL-1α ELISA kit
Rat Interleukin 1β,IL-1β ELISA Kit
Rat osteonectin,ON ELISA Kit
Rat Alpha-fetoprotein,AFP ELISA Kit
Rat annexin Ⅴ,ANX-Ⅴ ELISA Kit
Mouse eosinophil chemotactic factor,ECF ELISA Kit
Mouse soluble endothelial protein C receptor,sEPCR ELISA Kit
Mouse Interleukin 11,IL-11 ELISA KIT
Mouse Matrix metalloproteinase 2/Gelatinase A(MMP-2/Gelatinase A)ELISA Kit
Mouse Myoglobin,MYO/MB ELISA Kit
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Intron Biotechnology Inc.
PCR
i-Taq™ DNA Polymerase
(with Buffer, dNTPs) i-Taq™ PCR core Kit in high purity which is stable and effective
for DNA amplification regardless the sort and reaction condition of
template.
INT25022…………….........…………..……….……….…….….500unit
i-StarTaq™ DNA Polymerase
(with Buffer, dNTPs) i-StarTaq™ PCR core kit having hot-start function for reduction on
non-specific amplification and high specificity.
INT25162..………………………..……….……….…….….500unit
2xPCR Master mix Solution
2x PCR Master mix Solution is designed to get the best result with
simplicity that all components are premixed in a tube with 2x solution
mix type. INT25027(i-Taq)….………………...................................0,5 ml x 2 vials
INT25028(i-Taq)….……………………….........................1 ml x 5 vials
INT25166(i-StarTaq)……….……….................................0,5 ml x 2 vials
INT25186(i-pfu)….…………...…….…............….………..1 ml x 5 vials
FastMix/Frenche PCR Premix Kit
FastMix/FrencheTM PCR maximized sensitivity as well as amplification rate compared to thexisting Maxime series. By eliminating the
artificial additive, all components are optimized for performing PCR
and can be stable at room temperature up to months. INT25401.........………………………………………….......…96 Tubes
INT25411…………………………………………………....…96 Tubes
e-Myco™ Mycoplasma PCR Detection
Mycoplasma are commonand serious contaminants of cell cultures.
It has been shown that 30% to 87% of cell culturesare infected with
mycoplasma.
INT25235(detects 49 kind of mycoplasma).............................. 48 tubes
INT25237 (detects 209 kind of mycoplasma)......…….…….…48 tubes
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Intron Biotechnology Inc.
PCR
6X Loading Dye Solution (XB) or(XO)
6X Loading Dye Solution(XB) is used to prepare DNA markers and
samples for loading on agarose orpolyacrylamide gels. It is recommended for the routine DNA work use. Each Loading Dye contains
two tracking dye, orange G dye and xylene cyanol FF for visual tracking of DNA migration during electrophoresis. INT21161 (XB) ............….…………………………………………. 5 ml
INT21162 (XO) …..…………………………………………….……5 ml
SiZer™-DNA Marker Solution INT24071 (20 ).....……………………….…………..……..……0,5 ml
INT24072 (50 )….………………………..…………..……..……0,5 ml
INT24073 (100 )........………………….……………..……...…....0,5 ml
INT24074 (1000)......…………………..……………..….…..……0,5 ml
INT24076(15k)….…………………………………….……..……0,5 ml
RedSafe™ Nucleic Acid Staining Solution RedSafe™ Nucleic Acid Staining Solution (20,000x) is a new and
safe nucleic acid staining solution. It is an alternative to the traditional ethidium bromide(EtBr) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA.
INT21141.….…………………………………………………….0,5 ml
Other
M-MLV Reverse Transcriptase
M-MLV Reverse Transcriptase is one of DNA polymerase and
used for the synthesis of Single stranded RNA, DNA or complementary DNA strand from RNA:DNA hybrid like AMV RT.
INT27032……………………………..……….……….……10.000 Unit
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Intron Biotechnology Inc.
Other
AMV Reverse Transcriptase AMV Reverse Transcriptase is one of DNA polymerase and used for the synthesis of Single stranded RNA,
DNA or complementary DNA strand from RNA:DNA hybrid. This is commonly used for cDNA synthesis from RNA.
INT27021……………………………..…....…….…….…......…200 Unit
Proteinase K Proteinase K is prified from Tritirachium album and non specific serine protease which has activity at pH, salt,surface active agent and
temperature.
INT32181……………………………..…....…….….......1ml(30 mg/ml)
Ribonuclease A (RNase A) Ribonuclease A is endoribonuclease which is purified from bovine pancreas. This enzyme is useful to selectively remove RNA.
INT27062……………………………..…....…….……0,5 ml(10 mg/ml)
EtBr WiPER EtBr Wiper is an essential item for decontaminating Ethidium Bromide (EtBr) in the laboratory including the space, the surface of equipment, the surface of glassware, etc. INT21132……………………………..…....…….…….……2x200ml
www.biocem.com.tr
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Antigenix America Inc.
Super-X Plex® Flow Cytometry Cytokine Assays
Super-X Plex® Flow Cytometry Cytokine Assays
Create your own custom cytokine panels!
Select one or more assays from within each group/subcategory below.
Or Multiplex ACROSS GROUPS with custom design by our lab.
CUSTUMIZE YOUR MULTI-PLEX PANELS
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Antigenix America Inc.
Super-X Plex® Flow Cytometry Cytokine Assays
Or choose from our ready-made multi-plex cytokine panels below:
Human, Mouse, Rat, Non-Human Primate, or Canine
Human Th1/Th2/Th17 Panel(7-Plex):
Description/Target
Human Th1/Th2/Th17(7-Plex Panel)
Panel Description: Human IL-2
IL-4
IL-6
IL-10
IL-17A
INF Gamma
TNF ALPHA
ANTIGEN STANDARTS:
Human TH1/TH2/TH17 Panel
CAT#
HMX170
Super X Plex Kits Multi-Plex Panels
SMX170
Pre-Mixed- (For customers who need EXTRA Set of standards)
Human Inflammation Panel(7-Plex):
Description/Target
Human Inflammation(7-Plex Panel)
Panel Description: Human
IL-1 beta
IL-1 RA
IL-6
IL-8
IL-10
IL-12 p70
TNF ALPHA
ANTIGEN STANDARTS:
Human Inflammation Panel
www.biocem.com.tr
CAT#
HMX270
SMX270
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Cell Applications, Inc
Primary Cells
Cell Biology Products
www.biocem.com.tr
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Cell Applications, Inc
Media
Description
Cat. No.
Skeletal Muscle Cell Growth Medium
151-500
MesoEndo Cell Growth Medium
212-500
Endothelial Cell Growth Medium
211-500
Endothelial Cell Growth Medium Kit
211K-500
Smooth Muscle Cell Growth Medium
311-500
Smooth Muscle Cell Growth Medium Kit
311K-500
Synoviocyte Growth Medium
415-500
Human Mammary Epithelial Cell Growth Medium
815-500
Rat Brain Endothelial Growth Medium
R819-500
Cells
Description
Cat. No.
Short
Human Epidermal Keratinocytes
102-05a
HEK
Human Epidermal Keratinocytes, neonatal
102-05n
HEK
Human Coronary Artery Endothelial Cells, adult
300-05a
HCAEC
Human Coronary Artery Smooth Muscle Cells
350-05a
HCASMC
Human Aortic Smooth Muscle Cells, adult
354-05a
HAOSMC
Human Chondrocytes Normal Tissue, adult
402-05a
HC
Human Fibroblasts-Like Synoviocytes, Normal Tissue, adult
408-05a
HFLS
Human Fibroblasts-Like Synoviocytes, Normal Tissue, adult
408RA-05a
Human Fibroblasts-Like Synoviocytes, Normal Tissue, adult
602-05a
HFDPC
Human Mammary Epithelial Cells, adult
830-05a
HMEpC
Human Mammary Epithelial Cells, adult
R357-25
RCm
Rat Brain Microvascular Endothelial Cells, adult
R840-05a
RBMVEC
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HFLS-RA
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TONBO Biosciences
FCx-Pro™ Antibodies
SPECIES SPECIFICITY
FORMAT
TESTS
H
CD3
FITC/PE/Purified
25-100
H
CD3
APC/FITC/PE/PerCP-Cy5.5
25-100
H
CD4
APC/FITC/PE/PerCP-Cy5.5
25-100-500
H
CD19
APC/PE-Cy7/PE/PerCP-Cy5.5
25-100
H
CD25
APC/PE/PE-Cy7/Purified
25-100
H
CD45
APC/APC-Cy7/FITC/PE/PE-Cy7
25-100-500
H
CD44
APC/APC-Cy7/FITC/PE/PE-Cy7
25-100
H
IL-4
APC/In Vivo Ready/PE
25-100
H
IL-17A
APC/Alexa Fluor® 647
25-100
FCx-Product
PRODUCT
TESTS
Ghost Dye™ Red 780
100-500
Ghost Dye™ Violet 450
100-500
Ghost Dye™ Violet 450
100-500
Ghost Dye™ Violet 510
100-500
Ghost Dye™ Violet 510
100-500
Propidium Iodide
100-500
CFSE
500ug
7-AAD
200-500
Cell Stimulation Cocktail (500X)
100 µL
Flow Cytometry Perm Buffer (10X)
150 mL
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Assay Kits & Reagents
BioAssay Systems develops and markets innovative and high-throughput assay solutions to satisfy
the ever increasing demands of the life sciences industry. BioAssay Systems offers assay kits that
are simple and convenient to use, which are superior in performance and require little to no time for
assay optimization. We focus on safe, non-radioactive formats such as absorbance, fluorescence and
luminescence detection techniques.
PRODUCTS
CAT. NO.
QuantiChrom™ Acetylcholinesterase Assay Kit
DACE-100
QuantiChrom™ Calcium Assay Kit
DICA-500
EnzyChrom™ Ethanol Assay Kit
ECET-100
QuantiChrom™ Glucose Assay Kit
DIGL-100
QuantiChrom™ Uric Acid Assay Kit
DIUA-250
QuantiChrom™ Urea Assay Kit
DIUR-500
EnzyChrom™ Triglyceride Assay Kit
ETGA-200
QuantiChrom™ Zinc Assay Kit
DIZN-250
EnzyChrom™ Superoxide Dismutase Assay Kit
ESOD-100
EnzyChrom™ Nitric Oxide Synthase Assay Kit
ENOS-100
EnzyChrom™ Catalase Assay Kit
ECAT-100
QuantiChrom™ TBARS Assay Kit
DTBA-100
EnzyFluo™ ERK Phosphorylation Assay Kit
EERK-100
EnzyFluo™ NFKB Phosphorylation Assay Kit
ENFKB-100
QuantiChrom™ Iron Assay Kit
DIFE-250
EnzyChrom™ GSH/GSSG Assay Kit
EGTT-100
EnzyChrom™ Glutathione Peroxidase Assay Kit
EGPX-100
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G-Biosciences
Educational Products
Unlocking the Mysteries of Genetic Material
APLICATIONS
• Transformation of bacteria with a gene or DNA
• Understand simple molecular biology and genetic manipulation techniques
logy chno
e
• Role of bacteria as a production factory
t
o
un
Imm
• Understand transformation in biotechnology
Isolation & Characterization of Bacteria
APLICATIONS
• Cultivation of bacteria.
• Aseptic handling techniques.
• Bacteria isolation from test samples.
• Characterize bacteria with Gram staining.
• Applications of bacterial isolation
gy biolo
Micro
Polymerase Chain Reaction (PCR)
APLICATIONS
• Teaches the principle of polymerase chain reaction (PCR)
• Hands on PCR
• Contains everything necessary for amplification of a gene of interest
-­‐
cular
Mole ogy Biol
Enzyme Analysis
APLICATIONS
• Enzyme kinetic and regulation of enzyme activity.
• Role of substrate concentration.
• Effects of pH and temperature on the reaction rate.
• Inhibition of enzyme reaction.
d in an
Prote omic Prote
Immunoquantification Technique(ELISA)
APLICATIONS
• Teaches ELISA technique.
• Learn to perform ELISA assay and screen unknown samples.
• Simulates a real world ELISA test.
Im
logy chno
ote
mun
Supplied with components needed for hands-on experimentation for six workstations of 4-5
students or 24-30 students. Supplied with Teacher’s Guide and separate Student’s Guides.
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Santa Cruz Biotechnology, Inc.
Primary Antibodies
Santa Cruz Biotechnology now offers over 65.000 antibodies directed to a
broad range of mammalian and non-mammalian protein targets.
Bax An'body (N-­‐20): sc-­‐493 TLR2 An;body (S-­‐16): sc-­‐16237 p53 An'body (DO-­‐1): sc-­‐126 IRF-­‐1 Anibody (C-­‐20): sc-­‐497 β-­‐Ac'n An'body (C4): sc-­‐47778 α Tubulin An;body (YOL1/34): sc-­‐53030 CD45 An'body (H-­‐230): sc-­‐25590 PPARβ An;body (F-­‐7): sc-­‐74440 www.biocem.com.tr
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Santa Cruz Biotechnology, Inc.
Secondery Antibodies
Western Blotting
Conventional Secondary Antibodies
Isotype-Specific Secondary Antibodies
Cruz Marker Compatible Secondary Antibodies
Western Blotting
Control IgGs and IgG Conjugates
Control Immunoglobulins and Conjugates
IHC and Flow Cytometry
Secondary Antibodies
Conventional Secondary Antibodies
Isotype-Specific Secondary Antibodies
F(ab’)2 secondary antibodies
Cruzfluor™ Secondary Antibody Conjugates
IHC and Flow Cytometry
Secondary Antibodies
Normal Sera for Immunohistochemistry
WB Positive Control Cell Lysates • HeLa Cell Lysate
• A-431 Whole Cell Lysate
• Jurkat Cell lysate
Gene Silencer • Santa Cruz Biotechnology now offers siRNA and shRNA (plasmid and lentiviral) gene silencers targeted to all (100%)
known human and mouse genes
ImmunoCruz™
ImmunoCruz™ goat/mouse/rabbit LSAB Staining System
• System includes 15 ml each of negative control IgG, peroxidase blocking
solution, normal blocking serum, biotinylated secondary antibody and Avidin D-HRP reagent.
• Each of the above reagents is pre-diluted and ready to use for immunohistochemical staining of tissue sections.
• Also included are peroxidase substrate, DAB chromogen and DAB dilution
buffer.
• Number of slides: 150
ImmunoCruz™ rat/mouse/goat/rabbit ABC Staining System
•System includes 1.0 ml normal blocking serum, 250 µg biotinylated se-
condary antibody, avidin and biotinylated horseradish peroxidase (ABC reagents), peroxidase substrate, DAB chromogen and DAB dilution buffer.
•Tissue sections are first incubated with primary antibody. Biotinylated secondary antibody is added, followed by the addition of preformed ABC reagent. The target protein is visualized by incubation in peroxidase substrate.
•Number of slides: 200
www.biocem.com.tr
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Santa Cruz Biotechnology, Inc.
CRISPR/CAS9 Knockout Plasmids
CRISPR/Cas9 SYSTEM
The Clustered Regularly Interspaced Sorth Palindromic Repeats
(CRISPR)/Cas9 system is an adaptive immune defense mechanism used by Archea and bacteria for the degradation of foreign
genetic meterial.
This mechanism has been repurposed for orher functions,
including genomic engineering for mammalian systems, such as
gene knockout.
CRISPR/Cas9 PRODUCTS
Each (CRISPR)/Cas9 Knockout (KO) Plasmid product consists of a
pool o 3 plasmids designed to ensure identification and cleavage
of a specific gene for maximum knockout efficiency. Each plasmid
encodes a uniqe 20nt sequence derived from the GeCKO library.
•Cas9/gRNA complex binds to proto-spacer adjacent to the (PAM)
site and unwinds DNA.
•gRNA binds to target locus in genomic DNA adjacent to the PAM
site.
•Cas9 cleves the 5’exons of the gene targeted by the three gRNA
plasmids at three specific sites.
•Target gene is distrupted.
Santa Cruz Biotechnology offers HDR Plasmids specific to each CRISPR/Cas9 double
strand break site.
Santa Cruz offers CRISPR Double Nickase Plasmids !
Designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart.
Santa Cruz offers CRISPR/dCas9 Activation Plasmids !
Designed to activate gene expression.
Our CRISPR Plasmid product line now features:
• CRISPR/Cas9 Knockout Plasmids for a very efficient bi-allelic or mono-allelic gene KO
• HDR Plasmids for the homologous recombination i.e. repair of DNA DSB introduced by corresponding KO plasmids
• Cre Vector sc-418923 to excise the genetic material inserted by HDR
• CRISPR/dCas9 Activation Plasmids for transient activation/increase of gene transcription – 2 different products for 2
different target sequences ACT and ACT-2.
• Double Nickase Plasmids for a very specific knockout of gene expression with minimal off- Target effects – 2 different
products for 2 different target sequences offered NIC and NIC-2
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Santa Cruz Biotechnology, Inc.
Chamicals
Santa Cruz Biotechnology, Inc. now offers over 167,041 specialty biochemicals under our ChemCruz™ brand. Browse
our product line below, which is organized in categories based on Specific Protein Interaction (5,890 chemicals), General
Research Use (23,909 chemicals) or Chemical Class (158,333 chemicals). Individual chemicals may be found by searching
for a CAS number, product name or formula.
Biochemicals
Laboratory Chemicals
Dyes and Stains
Fluorescent Probes
Buffer Components
Solvents
Prepared Buffers
HRP and AP Substrates
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Santa Cruz Biotechnology, Inc.
Buffers
Dimethyl Sulfoxide (DMSO), cell culture reagent (CAS 67-68-5)
Phosphate Buffered Saline: 1 L of 10X
Tris Buffered Saline with Tween 20: 1 L of 10X
Tris Buffered Saline: 1 L of 10X
HEPES Buffer Solution (1M) (CAS 7365-45-9)
Tris Buffered Saline with Tween 20: 1 L of 20X
Phosphate Buffered Saline: 5 L of 20X
Ponceau S solution (CAS 6226-79-5)
Western Blot Stripping Buffer
Tris Acetate-EDTA buffer, 50X
BSA Blocking Buffer for ELISA
HEPES sodium salt solution (1M) (CAS 75277-39-3)
ChIP Wash Buffer
Tris-EDTA buffer solution
TRIS Glycine buffer solution
Laemmli Sample Buffer (odor-free), 2X
Laemmli Sample Buffer (odor-free), 2X
Tris Base solution (CAS 77-86-1)
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Santa Cruz Biotechnology, Inc.
LABWARE
UltraCruz™ Cell Scrapers, 40cm, sterile, 100/case: sc-213230
UltraCruz™ Cell Scrapers, 25cm, sterile, 100/case: sc-213229
UltraCruz™ Filter Flask, 150 ml, PES, 0.22 µm : sc-200309
UltraCruz™ Filter Flask, 150 ml, PES, 0.45 µm: sc-200310
UltraCruz™ Filter Flask, 250 ml, PES, 0.22 µm: sc-200253
UltraCruz™ Filter Flask, 250 ml, PES, 0.45 µm: sc-200254
UltraCruz™ Filter Flask, 500 ml, PES, 0.22 µm: sc-200255
UltraCruz™ Filter Flask, 500 ml, PES, 0.45 µm: sc-200256
UltraCruz™ Filter Flask, 1000 ml, PES, 0.22 μm: sc-359029
UltraCruz™ Filter Flask, 1000 ml, PES, 0.45 μm: sc-359028
UltraCruz™ Flask, Tissue Culture, 12.5 cm2, vent cap, :sc-200261
UltraCruz™ Flask, Tissue Culture, 25 cm2, vent cap, :sc-200262
UltraCruz™ Flask, Tissue Culture, 75 cm2, vent cap, :sc-200263
UltraCruz™ Flask, Tissue Culture, 182 cm2, vent cap, : sc-200264
UltraCruz™ Syringe Filter, PVDF, 0.10μm, 30mm, 30/case: sc-358810
UltraCruz™ Syringe Filter, PVDF, 0.22μm, 30mm, 30/case: sc-358812
UltraCruz™ Syringe Filter, PVDF, 0.45μm, 30mm, 30/case: sc-358814
Nylon Transfer Membrane, 0.45 µm, 30 cmx3 m roll
: sc-201701
Nitrocellulose Transfer Membrane, 0.22µm,30cmx3mroll :sc-3718
PVDF Transfer Membrane, 0.45µm,30cmx3mroll
:sc-3723
UltraCruz™ Tissue Culture Dish, 35 mm: sc-200284
UltraCruz™ Tissue Culture Dish, 60 mm: sc-351862
UltraCruz™ Tissue Culture Dish, 70 mm: sc-200285
UltraCruz™ Tissue Culture Dish, 100 mm: sc-200286
UltraCruz™ Petri Dish
35 mm : sc-351864
UltraCruz™ Petri Dish
60 mm : sc-351863
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Santa Cruz Biotechnology, Inc.
LABWARE
UltraCruz™ Tissue Culture Plates, 6 well, individually wrapped: sc-204443
UltraCruz™ Tissue Culture Plates, 12 well, individually wrapped: sc-204444
UltraCruz™ Tissue Culture Plates, 24 well, individually wrapped: sc-204445
UltraCruz™ Tissue Culture Plates, 48 well, individually wrapped: sc-204446
UltraCruz™ Tissue Culture Plates, 96 well, individually wrapped sc-204447
ExactaCruz™ Serological Pipet, 1ml, Individually Wrapped: sc-200275
ExactaCruz™ Serological Pipet, 2ml, Individually Wrapped: sc-200277
ExactaCruz™ Serological Pipet, 5ml, Individually Wrapped: sc-200279
ExactaCruz™ Serological Pipet, 10ml, Individually Wrapped: sc-200281
ExactaCruz™ Serological Pipet, 25ml, Individually Wrapped: sc-200283
ExactaCruz™ Serological Pipet, 50ml, Individually Wrapped: sc-213234
UltraCruz™ Centrifuge Tube, Conical, 15ml: sc-200250
UltraCruz™ Centrifuge Tube, Conical, 50ml: sc-200251
UltraCruz™ Single PCR Tube, 0,2 ml, pk: sc-205898
UltraCruz™ Microcentrifuge Tube, 0.5 ml, pk: sc-200269
UltraCruz™ Microcentrifuge Tube, 1.5 ml, pk: sc-200271
UltraCruz™ Microcentrifuge Tube, 2.0 ml, pk: sc-200273
UltraCruz™ Cryogenic Vials, Self Standing, PP, 1.2 ml : sc-204527
UltraCruz™ Cryogenic Vial, Self Standing, PP, 2 ml, : sc-204495
UltraCruz™ Cryogenic Vials, Self Standing, PP, 4 ml, : sc-204529
UltraCruz™ CryoBoxes
: sc:200201
ExactaCruz™ Serological Pipet, 1ml, Individually Wrapped: sc-200275
ExactaCruz™ Serological Pipet, 2ml, Individually Wrapped: sc-200277
ExactaCruz™ Serological Pipet, 5ml, Individually Wrapped: sc-200279
ExactaCruz™ Serological Pipet, 10ml, Individually Wrapped: sc-200281
ExactaCruz™ Serological Pipet, 25ml, Individually Wrapped: sc-200283
ExactaCruz™ Serological Pipet, 50ml, Individually Wrapped: sc-213234
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Biocem Sağlık Ürünleri San. Tic. Ltd. Şti.
Hizmetlerimiz
Real-Time PCR Hizmeti
Laboratuvarımızda qPCR uygulamalarında Applied Biosystems® marka 5 filtreye sahip ( FAM/SYBR Green, VIC/JOE, NED/TAMRA/Cy3, ROX/Texas
Red ve Cy5) 10-30 ul reaksiyon hacmiyle çalışmaya uygun 7500 Fast cihazı kullanılmaktadır. Laboratuarımızda aşağıdaki real-time uygulamaları konusunda hizmet verilmektedir:
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Kalitatif analizler (İnternal pozitif kontrol kullanılarak var (plus) / yok (minus) analizleri)
Absolute ve Relatif Gen Miktar Tayini
Gen Ekspresyonu Analizi (ΔΔCT yöntemi)
miRNA Gen Expresyon Analizi
Genetik Varyasyon Analizi: SNP/CNV Genotipleme, İlaç Metabolizması Genotipleme,
High-Resolution Melt Curve (HRM)
PCR Hizmeti
Laboratuarımızda bulunan Applied Biosystems® marka Veriti® 96-Well Thermal Cycler ve ProFlex™ PCR Sistemi (3x32-Well) cihazlarımız ve
MiniBISPro jel görüntüleme cihazımız ile PCR, touch down PCR ve optimizasyon çalışmaları konusunda hizmet verilmektedir.
Dizileme ve Fragman Analizi Hizmeti
Dizileme ve Fragman Analizi konusunda Applied Biosystems®marka, 8 kapilerli 3500 cihazımız ile hızlı ve kaliteli sonuç alabilmekteyiz. Fragman
ve dizi analizlerinde aşağıda verilen programlar ile güvenilir analiz hizmeti verilmektedir:
• Sequencing Analysis Software
• Sequencing – SeqScape® Software v2.7
• Fragment analysis – GeneMapper® Software v4.1
Ayrıca özel gen bölgeleri için dizi ve fragman primer dizaynı ve proje danışmanlık hizmeti verilmektedir.
Fragman Analizi
1200 baz çiftine kadar hassas ve güvenilir fragment analizi yapılabilmektedir. Bu kapsamda aşağıdaki çalışmalar yapılmaktadır:
• Microsatellite (STR) analizi
• SNP Genotipleme
• Fingerprinting
• Relative Fluorescence
Fragman analizi için florasan boya seçenekleri:6-FAM, VIC, NED, PED, LIZ (STANDART)
Dizi Analizi
Applied Biosystems®marka kullandığımız BigDye® Terminator v3.1 Cycle Sequencing Kit ile çift yönlü dizilemede 1000 baz çiftine kadar güvenilir ve
hızlı dizi analizi hizmeti verilmektedir (QVs>20). Örneklerin hangi aşamadan itibaren işlem göreceğini (DNA izolasyonu-PCR-Cycle Sequencing-Dizileme ) bize bildirmeniz yeterlidir.
Yeni Nesil Sekanslama Hizmeti
Nükleotid bağlanması sırasında salınan hidrojen iyonlarının kullanarak bazların belirlenmesini sağlayan Ion Torrent™ sistemi ile istenilen
çalışmaya uygun olan çipler (314, 316 ve 318) kullanılarak laboratuarımızda aşağıda yer alan hazır paneller veya kullanıcının isteğine özel genler ile
oluşturulan dizayn paneller (Ion Community Panels) ile hizmet verilmektedir:
Ion AmpliSeq Ready-to-use Panels;
• Ion Ampliseq RNA Apoptosis Panel
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Ion Ampliseq RNA Cancer Panel
Ion Ampliseq Cancer Hotspot Panel v2
Ion Ampliseq Comprehensive Cancer Panel
Ion Ampliseq Inherited Disease Panel
Ion Ampliseq Cancer Panel Primer Pool
Ion AmpliSeq Community Panels;
• Ion Ampliseq CFTR Panel
• Ion Ampliseq TP53 Panel
• Ion Ampliseq BRCA1 ve BRCA2 Panel
• Ion Ampliseq Colon ve Lung Cancer Panel
• Dementia Research Gene Panel
• Ion Ampliseq Human Identity Panel
• Ion Ampliseq AML Panel ( gelecek! )
• Ion Ampliseq Cardio Panel ( gelecek!)
Ayrıca yukarıdaki paneller dışında istenen gen bölgelerine ait panellerin custom olarak dizayn edilerek çalışılması imkanı da bulunmaktadır.
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Biocem Sağlık Ürünleri San. Tic. Ltd. Şti.
Notlar
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Biocem Sağlık Ürünleri San. Tic. Ltd. Şti.
Notlar
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