rtf,l - İzmir Katip Çelebi Üniversitesi

İzmir
T.c.
Kitip Çetebi Üniversitesi
Bilimsel Araştırma Projeleri Koordinatörlüğü
TEKL|F isrervıe MEKTUBU
Tel: 0 232 329 35
İhale
No
:
:
35
Fax:O 232 325 33 55
1
37
8.03.20l6
İlgiliairim BilimselAraştırmaProjeleriKoordinatörlüğü
Üniversitemiz Tıp Fakültesi öğretim üyesi Yrd. Doç. Dr. Zeki SOYPAÇACl tarafından yürütülen 2016-TDUlşin Adı
: T|PF-0015 numaralı Pauci -İmmun Glomerulonefrit Tanılı Hastaların boOrel< iğne Biyopsilerinde Mtor
Yolağının Rolü için 5 kalem malzeme alımı.
iı_ciı-i FlRMAyA,
idaremizce, 5 Kalem Mal Alımı lşine ait aşağıdaki mal/hizmetin
fiyatlarına ihtiyaç vardır. Listedeki kalemlere fiyat vermenizi rica olunur.
ooĞnuoııı ıeıvıiıı
nirim
.,
"/rtf,l
Sıra
No
1
Malzeme / Hizmet Adı
LABORATUVAR DiGER
MALZEMELERl (mTOR lam
bovası-1 adet -
2
3
4
5
Adet
1
Adet
1
Adet
1
Kutu
1
Kutu
3
Açıklama
Marka
Birim Fiyat
(KDV Haric)
Toplam
TUTAR
)
LABORATUVAR DiĞER
MALZEMELERl (Mikrotom
bıçağı -
Miktar
)
LABORATUVAR DiĞER
MALZEMELERi (PTEN lam
boyası-1 adet - )
LABORATUVAR DiGER
MALZEMELERi (TGF-beta lam
bovası -
Birim
)
LABORATUVAR DiĞER
MALZEMELERl (Lizinli lam
Para Birimi; Tl'dir. Fiyatınızı KDV hariç olarak, TL
FÜRK Lirası) cinsinden
veriniz.
1-Teklifinizi en geç 25.03.2016 SAAT 17:30 a kadar Bilimse| Araştırma Projeleri Koordinatörlüğü, SATIN ALMA
birimine teslim ediniz veya fax ile iletiniz. Tekliflerinizi size gönderilen Matbu form üzerine yazmanz (proforma üzerine
yazılmaması) rica olunur.
2 - Teklifinizi KDV hariç, birim fiyat olarak veriniz. Teklif mektubu ve teknik şartnamenin her sayfasını kaşeleyip imzalayınız. Varsa
markasını mutlaka yazınız.
3
-
Doğrudan Temin Usulü ile alım yapılması halinde aşağıdaki şartlar geçerli olacaktır.
3.1 - Teklif mektubunda vermiş olduğunuz fiyatlar, şartnamede aksine bir süre yoksa Otuz (3O) gün süreyle geçerli olacaktır.
3.2 - Taahhüt edilen mal/veya hizmet, ekli şartnamelerde aksine bir süre yoksa tebliğ tarihinden itibaren en 9eç .:,ı ı.](.]:, ] :;, takvim günü
içinde ilgili Birime teslim edilecektir.
Bu sürenin bitiminde taahhüdün yerine getirilmemesi durumunda idare tek taraflı olarak alımdan vazgeçebilir.
3.3 - Teklif veren firmalar, yukandaki şartlarla birlikte, varsa; teknik şartname ve
/
veya numunedeki özellikleri aynen kabul etmiş sayılır.
4- Ödeme Şartları: Ödenek Durumuna Ve Faturanln Saymanlığa antikal ediş slraslna göre (tahminen otuz gün içinde) yapllacaktlr.
5- Teklif mektubumuz
size ulaştığı takdirde, olumlu ya da olumsuz yazılı olarak geri bildirim yapnız. (Elden veya faks ile)
Adres: İzmir Katip Çelebi Üniversitesi Çiğti Ana Yerleşkesi 35620 Çiğli/İzmir - TÜRKiyE
iı_oiı_i FIRMANıN;
Adı / Ünvanı:
Adres:
TeI:
E1
126RUo
Rev E
Page 1 of
'!
SPRrrV6
BloSclENcE
Rabbit Anti-Human Transformlng Growth Factor beta1 (TGFB1) Polyclonal
Antibody
CATALOG
El1260
0.1 ml immunogen affinity purified rabbit polyclonal
antibody in PBS/1% BSA buffer pH 7.6 with less
than 0.1% sodium azide.
E11262
0.5 ml immunogen affinity purified rabbit polyclonal
antibody in PBS/1% BSA buffer pH 7.6 with less
than 0.1% sodium azide.
E11264
1.0 ml immunogen affinity purified rabbit polyclonal
antibody in PBS/1% BSA buffer pH 7.6 with less
than 0.1% sodium azide.
E11261
7.0 ml pre-diluted immunogen affinity purified rabbit
polyclonal antibody in TBS/'1% BSA buffer pH 7.6
with less than 0.1% sodium azide.
#:
lNTENoED usE:
CLONE:
lMMUNOGEN:
lG ISOWPE:
EPlTOPE:
MOLECULAR WElGHT
SPEClES REACTIV|W:
DESGRlPTlON:
APPLıcATloNs:
lHC PROCEDURE:
ı"
. n' 1.1
Human placenta Stained with
anti-TGF B1 antibody
For Research Use Only. Not for use in diagnostic procedures.
N/A
Recombinant protein encoding human TGFP1 aa 177-391
Rabbit lgG
Not determined
12.5/39kDa
Human (tested). (See www.sorinqbio.com for informatıon on species reactivity predicted by
sequence homology.)
A total of three members of the TGF beta family, TcFbetal, TGFbeta2 and TGF beta3, have been
identified in mammals. TGF betas mediate their activity by high affini§ binding to the type ll
receptor transmembrane protein with a cytoplasmic serinepthreonine kinase domain.
lmmunohistochemistry
(lHC)
Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this
primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
AntibodyDilution: lfusingtheconcentrateformatofthisproduct,dilutetheantibody1:25.The
dilutions are estimates; actual results may differ because of variabili§ in methods and protocols.
Antigen Retrieval: Boil tissue section in 1OmM citrate buffer, pH 6.0 for 10 min followed by
cooling at RT for 20 min.
Primary Antibody lncubation: lncubate for 30 minutes at RT.
Stide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween.
Visualization: Detect the antibody as instructed by the instructions provided
with the visualization
syStem.
POSIT|VE CONTROL:
Placenta
CELLULAR LOGAL|ZAT|ON:
SToRAGE & sTABlLlw
Cytoplasm, membrane
store at 2-8'c. Do not freeze. The user must validate any other storage conditions. When properly
stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the
expiration date.
There are no definitive signs to indicate instability of this product; therefore, positive and negative
controls should be tested simultaneously with unknown specimens.
lf unexpected results are observed which cannot be explained by variations in laboratory
procedures and a problem with the reagent is suspected, contact Technical Support at
[email protected].
wARNlNGs &
PRECAUTİONS:
4300 Hacienda Drive
Pleasanton, CA 94588
www. sorinobio.com
1.
2.
3.
4.
Avoid contact of reagents with eyes and mucous membranes. lf reagents come into contact
with sensitive areas, wash with copious amounts of water.
This product is harmful if swallowed.
Consult local or state authorities with regard to recommended method of disposal.
Avoid microbial contamination of reagents.
,§§,§',rrd
,_.,}l,iitı
rel: 1-925-474-8440
Toll Free: 1 -800-787-6896
Fax 1-925-474-8469
M470RUo
Rev D
Page 2 oi 2
WESTERN BLOTT|NG
POSlTlVE CONTROL:
FLOW CYTOMETRY:
FLOW CYTOMETRY
MCF-7 Ce|l Lysate
Recommended starting protocol: Dilute the antibody 1:400. lncubate for 30 minutes at 4"C.
The dilution is an estimate; actual results may differ because of Variability in methods and protocols,
optimal dilution and procedure should be determined by the end user.
POSIT|VE GONTROL:
4431 cell Line
CELLULAR LOCAL|ZAT|ON:
Cytoplasm
STORAGE & STAB|LITY:
wARNlNGs &
PRECAUT|ONS:
4300 Hacienda Drive
Pleasanton, CA 94588
www. sorinobio.com
Store at 2_8'C, Do not freeze, The user must validate any other storage conditions. When properly
stored, the reagent is Stable to the date indicated on the label. Do not use the reagent beyond the
expiration date.
There are no definitive signs to indicate instability of this product; therefore, positive and negative
controls should be tested simultaneously with unknown specimens.
lf unexpected results are observed which cannot be explained by variations in laboratory
procedures and a problem with the reagent is suspected, contact Technical Support at
[email protected].
,l
Avoid contact of reagents with eyes and mucous membranes. lf reagents come into contact
with sensitive areas, wash with copious amounts of water.
This product is harmful if swallowed.
Consult local or stale authorities with regard to recommended method of disposal.
Avoid microbial contamination of reagents.
.
2.
3.
4.
re| 1-925-474-8440
Toll Free: 1-800-787-6896
Fax: 1-925-474-8469
M470RUo
Rev D
Page 1 ot 2
_*iF{ry.q
Rabbit Anti-Human PTEN Monoclonal Antibody (CIone
ıD.
CATALOG
0.1 ml rabbit monoc|onal antibody purified
by protein A/G in PBS/1% BSA buffer pH
7.6 with less than 0.1% sodium azide.
#:
ı@-
M4702 0.5 ml rabbit monoclonal antibody purified
by protein Aı/G in PBS/1% BSA buffer pH
7.6 with less than 0.1% sodium azide.
M4704
1.0 ml rabbit monoclonal antibody purified
by protein A/G in PBS/1% BSA buffer pH
7.6 with less than 0.1% sodium azide.
adenocarcinoma stained
with anti-PTEN antibody
M4701 7.0
ml pre-diluted rabbit monoclonal
antibody purified by protein A,/G in TBS/1%
BSA buffer pH 7.6 with less than 0.1 %
sodium azide.
:
5:A
l\
-,- /\
.l_J___\./
western Blot
analysis of
MCF-7 cell
lysate with
PTEN
antibody.
l\
/\
^\
_
H:J;r:ff!;;ıi'!;:;,";
antibody in A431 (green)
compare to negative control
of rabbit
lgc (blue)
For Research Use Only. Notfor use in diagnostic procedures.
lNTENDED USE:
CLONE:
sP"|70
lMMUNOGEN:
lG ISOTYPE:
Synthetic peptide close to the N-terminus of human PTEN protein.
Rabbit lgG
EPlTOPE:
MOLECULAR WElGHT:
Not determined
SPEClES REACT|V|TY:
Human (tested). (See www.sprinob|o.com for information on species reactivity predicted by
sequence homology.)
Phosphatidylinosito|_3,4, 5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase
(PTEN) is a tumor suppressor and a member in the Pl3I(PTEN/AKT pathway. PTEN is a
phosphatase with dual specificity to slow cell groMh and antagonize the growth promoting activities
from HER protein kinases. The defects of PTEN, mostly around the C{erminus of PTEN, have been
implicated in many human cancers and diseases. Therefore, antibody to the N terminus will not be
ab|e to detect most of the PTEN protein loss.
lmmunohistochemistry (lHC), Western Blotting and Flow cytometry
DESCRlPTlON:
APPLlGATlONS:
lHG PROCEDURE:
54 kDa
Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this
primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: lf using the concenlrate format of this product, dilute the antibody 1:100. The
dilutions are estimates; actual results may differ because of variability in methods and protoco|s.
AntigenRetrieval: Boil tissuesectioninEDTAbuffer,pH8.0forlOminfol|owedbycoolingat
room temperature for 20 min.
Primary Antibody lncubation: lncubate for 10 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization
system.
lHc PoslTıVE coNTRoL:
prostate Adenocarcinoma
WESTERN BLOTllNG:
Recommended starting protocol: Dilute the antibody 1:25. lncubate for 1 hour at room temperature.
The di|ution is an estimate; actual results maydiffer because of variability in methods and protocols.
Optimal dilution and procedure should be determined by the end user.
4300 Hacienda Drive
Pleasanton, CA 94588
www.sprinobio.com
i}i*'
Te| 1-925-474-8440
Toll Free: 1-800-787-6896
Fax: 1-925-474-8469
E1859RUo
Rgv E
Paga 2 oİ 2
WARNlNGs &
PREcAuTloNs:
1,
2,
3.
4.
4300 Hacienda DriVe
Pleasanton, cA 94588
www.sorinobio.com
Avoid contact of reagents with eyes and mucous mombranos, lf reagents come into contact
with sansitive areas, wash with copious amounts of wat€r.
This product is harmful if swallowed,
consull local or state aı]thorities with regard to recommended method of disposal,
Avoid microbial contamination of rcagents,
re| |92'47 4-8440
Toll Free: 1-800-787-6896
Fax: 1-92 74-8469
E1859RUo
ReV E
Paga 1 of 2
-ÇeF{ryg
ffi
yffi
Rabbit Anti-Human mTOR Polyclonal Antibody
cATALoG f:
E'l8590
0.1 ml ammunogen affinily purifiad labbit polyclonal
antibody in PBs/1% BsA buffer pH 7.6 with Iess
than 0.1% sodium azide.
El8592
o.5 ml immunogen affinity purjfied rabbit polyclonal
antibody in PBs/l% BsA buffer pH 7.6 with les§
than 0.1% sodium azide.
Et8594
1.o ml immunogen affinity purified rabbit polyclonal
antibody in PBs/1% BsA buffer pH 7.6 with less
lhan 0.1"/o sodium azide.
E18591
7.o ml pre-diluted immunogen atinity pürrified rabbit
polyclonal antibody in TBs/1% BsA bufier pH 7.6
with less than 0.1% sodium azide,
,..
For Research Use only. Not for use in diagnostic procedures,
cLoNEI
N/A
Synthetic peplide derived from c-terminus of human mToR protein.
Rabbit lgG
lG lsoTYPEI
EPIToPE:
MOLECULAR WElGHT
sPEclEs REAcTlv|TY:
DEscRlPTloN:
APPL|cATloNs:
lHc PRocEoURE:
'.',-{'^
Human breasI carcinama
w ith anti -mTo R antibody
lNTENDED usE:
lMMUNoGEN:
ı , tI
Not determined
289kDa
Human (lested). (See u4^4!§grj!gho.ç9E for information on species reactivity predicted by
sequence homology,)
mToR, o. FKBP12 rapamycin associated protein (FRAP), is one of a family of proteins involved in
cellcycle progression, DNA reoombination, and DNA damage deteclion. ln ral, it is a 289kDa
prolein (symb;lized RAFTl) wilh significant homology to ihe saccharomyces ce,evisiae protein
ioR1 a;; has been shown |o associate with the imrnunophilin FKBP12 in a rapamycin dependent
fashion, The mToR-rapamycin complex is known io inhibit progression through the G1 cell cyc]e
jn several cell
stage by interfering wit'h miiogenic signaling palhways involved in G1 progression
ıypÖs, js well as in yeast, Th; binding of FRAP to FKBP12,rapamycin correlate with the abi|ity of
these ligands to inhibit cellcycle progression.
(lHc)
specimen Preparation: Formalin-fixed, paraffin-embeddod tissı]es are suitab|e for use with ihls
lmmunohistochemistry
primary antibody.
beparifiinızation: Deparaffinize slides using xylene or xylene alternative and graded alcohols,
Aniiuoay oilution, lf ıJsing lhe concentrate fomat of this product, dilute the antibody 1:50. The
dilulions;re estimates; actual results may diffel because of Variability in methods and plotocols,
Antigon Retİieval: Boil tissue section in 1omM citrate buffer, pH 6.0 for 10 min followed by
cooling at room temperature for 20 min.
Primary Antibody lncubation: lnclbate for 30 minltes at room temperature.
slido tİashlng: stldes must b€ washed in between steps. Rinse slides with PBs/o.05% Tween.
by the instructions provided with lhe visualization
system.
Breast carcinoma
Membrane, cyloplasm
slore at 2-8'C. Do not freeze. The user must Validate any other storage conditions. when ploperly
stored, the reagent is slable to lhe date indicated on the label. Do not use lhe reagent beyond the
visualizatlona Detect the antibody as instructed
PoslTlvE coNTRoL:
cELLuLAR LocALlzATloN:
sToRAGE
&
sTABlLlTY
oxpiration date.
There are no derlnilive signs lo indıcate instability ofthis product; thelefore, positive and negalive
controls should be tested simultaneously wilh unknown specimens.
lf unexpected results are observed which cannot be explained by Varialions in laboralory
procedures and a problem with the reagent rs suspected, conlacl Technical support aı
[email protected].
4300 Hacienda Drive
Pleasanlon, cA 94588
www.sorinobio.com
re| |92ç474-u40
Toll Free: 1-800-787-6896
Fax:
1-92u74-u69